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Literature summary extracted from

  • Yupsanis, T.; Symeonidis, L.; Kalemi, T.; Moustaka, H.; Yupsani, A.
    Purification, properties and specificity of an endonuclease from Agropyron elongatum seedlings (2004), Plant Physiol. Biochem., 42, 795-802.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.30.2 Ca2+ 50% inhibition Thinopyrum elongatum
3.1.30.2 Cu2+ slight inhibition Thinopyrum elongatum
3.1.30.2 DTT slight inhibition Thinopyrum elongatum
3.1.30.2 Mn2+ slight inhibition Thinopyrum elongatum
3.1.30.2 additional information no inhibition by NEM and iodoacetic acid Thinopyrum elongatum
3.1.30.2 NaCl slight inhibition Thinopyrum elongatum
3.1.30.2 Ni2+ slight inhibition Thinopyrum elongatum
3.1.30.2 putrescine
-
 Thinopyrum elongatum
3.1.30.2 spermidine
-
 Thinopyrum elongatum
3.1.30.2 spermine 70% inhibition at 0.1 mM Thinopyrum elongatum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.30.2 additional information no effect by Mg2+ Thinopyrum elongatum
3.1.30.2 Zn2+ stimulates Thinopyrum elongatum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.30.2 34000
-
 1 * 34000, SDS-PAGE Thinopyrum elongatum
3.1.30.2 36000
-
 gel filtration Thinopyrum elongatum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.30.2 dsDNA + H2O Thinopyrum elongatum hydrolysis of nucleic acids ?
-
 ?
3.1.30.2 additional information Thinopyrum elongatum substrate specificity in descending order is ssDNA, dsDNA, RNA ?
-
 ?
3.1.30.2 RNA + H2O Thinopyrum elongatum hydrolysis of nucleic acids ?
-
 ?
3.1.30.2 ssDNA + H2O Thinopyrum elongatum hydrolysis of nucleic acids, preference for ssDNA ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.30.2 Thinopyrum elongatum
-
 8x = Elytrigia turcica
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.30.2 native enzyme 265fold to homogeneity from seedlings by ammonium sulfate fractionation, anion exchange chromatography and heparin affinity chromatography Thinopyrum elongatum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.30.2 seedling
-
 Thinopyrum elongatum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.30.2 additional information
-
 purified enzyme Thinopyrum elongatum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.30.2 5'-CCTGGCAGTT + H2O synthetic labeled deoxydecanucleotide, cleavage of 5'-Ap*G bond and with lower activity of Gp*T, Cp*A, and Gp*G bonds Thinopyrum elongatum ?
-
 ?
3.1.30.2 dsDNA + H2O hydrolysis of nucleic acids Thinopyrum elongatum ?
-
 ?
3.1.30.2 additional information substrate specificity in descending order is ssDNA, dsDNA, RNA Thinopyrum elongatum ?
-
 ?
3.1.30.2 additional information substrate specificity, no activity with dinucleoside monophosphates, overview Thinopyrum elongatum ?
-
 ?
3.1.30.2 poly(I) + H2O synthetic homopolyribonucleotide Thinopyrum elongatum ?
-
 ?
3.1.30.2 polyA + H2O synthetic homopolyribonucleotide Thinopyrum elongatum ?
-
 ?
3.1.30.2 polyC + H2O synthetic homopolyribonucleotide Thinopyrum elongatum ?
-
 ?
3.1.30.2 polyG + H2O synthetic homopolyribonucleotide Thinopyrum elongatum ?
-
 ?
3.1.30.2 polyU + H2O synthetic homopolyribonucleotide Thinopyrum elongatum ?
-
 ?
3.1.30.2 RNA + H2O hydrolysis of nucleic acids Thinopyrum elongatum ?
-
 ?
3.1.30.2 ssDNA + H2O hydrolysis of nucleic acids, preference for ssDNA Thinopyrum elongatum ?
-
 ?
3.1.30.2 supercoiled plasmid DNA + H2O
-
 Thinopyrum elongatum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.30.2 monomer 1 * 34000, SDS-PAGE Thinopyrum elongatum

Synonyms

EC Number Synonyms Comment Organism
3.1.30.2 endonuclease
-
 Thinopyrum elongatum
3.1.30.2 nuclease A
-
 Thinopyrum elongatum
3.1.30.2 plant type I nuclease
-
 Thinopyrum elongatum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.30.2 37
-
 assay at Thinopyrum elongatum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.30.2 60
-
 10 min, loss of 30% of activity Thinopyrum elongatum
3.1.30.2 70
-
 10 min, loss of 90% of activity Thinopyrum elongatum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.30.2 5.5
-
-
 Thinopyrum elongatum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.30.2 Thinopyrum elongatum isoelectric focusing
-
 4.7