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Literature summary extracted from

  • Minic, Z.; Rihouey, C.; Do, C.T.; Lerouge, P.; Jouanin, L.
    Purification and characterization of enzymes exhibiting beta-D-xylosidase activities in stem tissues of Arabidopsis (2004), Plant Physiol., 135, 867-878.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.37 gene xyl1, DNA and amino acid sequence determination and analysis, expression and phylogenetic analysis Arabidopsis thaliana
3.2.1.37 gene xyl4, DNA and amino acid sequence determination and analysis, expression and phylogenetic analysis Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.37 0.7
-
 4-nitrophenyl-beta-D-xylopyranoside pH 5.0, 37°C Arabidopsis thaliana
3.2.1.37 1.2
-
 4-nitrophenyl-beta-D-xylopyranoside pH 5.0, 37°C Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.37 64000
-
 x * 64000, SDS-PAGE Arabidopsis thaliana
3.2.1.37 67000
-
 x * 67000, SDS-PAGE Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.37 Arabidopsis thaliana Q9FGY1
-
-
3.2.1.37 Arabidopsis thaliana Q9FLG1
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.37 glycoprotein binds to concanavalin A Arabidopsis thaliana

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.37 native enzyme from stem, 29.1fold by concanavalin A affinity chromatography, cation exchange chromatography, and gel filtration to homogeneity Arabidopsis thaliana
3.2.1.37 native enzyme from stem, 40.3fold by concanavalin A affinity chromatography, cation exchange chromatography, and gel filtration to homogeneity Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.37 stem
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.37 4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
 Arabidopsis thaliana 4-nitrophenol + alpha-L-arabinofuranose
-
 ?
3.2.1.37 4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Arabidopsis thaliana 4-nitrophenol + beta-D-xylose
-
 ?
3.2.1.37 additional information xylotriose is a poor substrate Arabidopsis thaliana ?
-
 ?
3.2.1.37 additional information xylotriose is a poor substrate, the bifunctional enzyme shows two catalytic activities: alpha-L-arabinofuranosidase and beta-D-xylosidase activities Arabidopsis thaliana ?
-
 ?
3.2.1.37 xylobiose + H2O
-
 Arabidopsis thaliana 2 beta-D-xylose
-
 ?
3.2.1.37 xylotetraose + H2O
-
 Arabidopsis thaliana xylotriose + beta-D-xylose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.37 ? x * 67000, SDS-PAGE Arabidopsis thaliana
3.2.1.37 ? x * 64000, SDS-PAGE Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
3.2.1.37 alpha-arabinofuranosidase/beta-xylosidase
-
 Arabidopsis thaliana
3.2.1.37 beta-D-xylosidase
-
 Arabidopsis thaliana
3.2.1.37 More cf. EC 3.2.1.55, alpha-L-arabinofuranosidase Arabidopsis thaliana
3.2.1.37 XYL1
-
 Arabidopsis thaliana
3.2.1.37 XYL4
-
 Arabidopsis thaliana
3.2.1.37 xylosidase
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.37 55
-
-
 Arabidopsis thaliana
3.2.1.37 60
-
-
 Arabidopsis thaliana

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.37 20 70
-
 Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.37 1.35
-
 4-nitrophenyl-beta-D-xylopyranoside pH 5.0, 37°C Arabidopsis thaliana
3.2.1.37 1.65
-
 4-nitrophenyl-beta-D-xylopyranoside pH 5.0, 37°C Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.37 5
-
 assay at Arabidopsis thaliana