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Literature summary extracted from

  • Sharma, N.; Park, S.W.; Vepachedu, R.; Barbieri, L.; Ciani, M.; Stirpe, F.; Savary, B.J.; Vivanco, J.M.
    Isolation and characterization of an RIP (ribosome-inactivating protein)-like protein from tobacco with dual enzymatic activity (2004), Plant Physiol., 134, 171-181.
    View publication on PubMedView publication on EuropePMC

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.2.22 26000
-
 x * 26000, SDS-PAGE Nicotiana tabacum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.22 additional information Nicotiana tabacum the enzyme inhibits translation of rabbit reticulocytes and wheat germ lysate, the enzyme shows anti-microbial activity, e.g. against Trichoderma reesei, Ervinia amylovora, Shigella asonei, Salmonella typhimurium, Rhizobium leguminosarum, Cytospora cankar, and Pseudomonas solancearum, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.22 Nicotiana tabacum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.22 native enzyme from leaves by ion exchange chromatography and gel filtration Nicotiana tabacum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.2.22 leaf
-
 Nicotiana tabacum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.2.22 additional information
-
 adenine releasing activity with substrates hering sperm DNA, rRNA, and tRNA, depurination activity with ribosome substrate from Artemia sp. and rat Nicotiana tabacum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.22 Artemia sp. ribosome + H2O depurination of rRNA Nicotiana tabacum ?
-
 ?
3.2.2.22 hering sperm DNA + H2O adenine releasing activity Nicotiana tabacum ?
-
 ?
3.2.2.22 additional information the enzyme inhibits translation of rabbit reticulocytes and wheat germ lysate, the enzyme shows anti-microbial activity, e.g. against Trichoderma reesei, Ervinia amylovora, Shigella asonei, Salmonella typhimurium, Rhizobium leguminosarum, Cytospora cankar, and Pseudomonas solancearum, overview Nicotiana tabacum ?
-
 ?
3.2.2.22 rat ribosome + H2O depurination of rRNA Nicotiana tabacum ?
-
 ?
3.2.2.22 tRNA + H2O adenine releasing activity Nicotiana tabacum ?
-
 ?
3.2.2.22 yeast ribosome + H2O depurination of rRNA Nicotiana tabacum ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.2.22 ? x * 26000, SDS-PAGE Nicotiana tabacum
3.2.2.22 More the N-terminal amino acid sequence of the enzyme is identical with that of several superoxide dimutases Nicotiana tabacum

Synonyms

EC Number Synonyms Comment Organism
3.2.2.22 (ribosome-inactivating protein)-like protein
-
 Nicotiana tabacum
3.2.2.22 TRIP
-
 Nicotiana tabacum
3.2.2.22 type I RIP
-
 Nicotiana tabacum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.22 37
-
 assay at Nicotiana tabacum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.2.22 4 7 the pH preference depends on the substrate type, overview Nicotiana tabacum