Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Dehesh, K.; Tai, H.; Edwards, P.; Byrne, J.; Jaworski, J.G.
    Overexpression of 3-ketoacyl-acyl-carrier protein synthase IIIs in plants reduces the rate of lipid synthesis (2001), Plant Physiol., 125, 1103-1114.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.180 expression of the enzyme in transgenic Nicotiana tabacum using the Agrobacterium tumefaciens transfection system Spinacia oleracea
2.3.1.180 isozymes KAS III-1 and KAS III-2, DNA and amino acid sequence determination and analysis, seed-specific expression of the isozymes in transgenic Nicotiana tabacum, Brassica napus, and Arabidopsis thaliana using a napin gene promoter and the Agrobacterium tumefaciens transfection system Cuphea hookeriana
2.3.1.180 seed-specific expression of the isozymes in transgenic Nicotiana tabacum, Brassica napus, and Arabidopsis thaliana using a napin gene promoter and the Agrobacterium tumefaciens transfection system Brassica napus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.180 additional information analysis of fatty acid composition and acyl-ACP content of transgenic plants expressing the two isozymes of Cuphea glaucescens, the transgenic plants show altered fatty acid levels with accumulation of palmitate Cuphea hookeriana
2.3.1.180 additional information transfection with Cuphea glaucescens isozymes KAS III-1 and KAS III-2 leads to altered fatty acid levels with accumulation of palmitate Brassica napus
2.3.1.180 additional information transfections with Cuphea glaucescens isozymes KAS III-1 and KAS III-2 or the spinach enzyme lead to altered fatty acid levels with accumulation of palmitate Nicotiana tabacum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.180 42000
-
 x * 42000, about, amino acid sequence calculation, isozyme KAS III-1, x * 43000, about, amino acid sequence calculation, isozyme KAS III-2 Cuphea hookeriana
2.3.1.180 43000
-
 x * 42000, about, amino acid sequence calculation, isozyme KAS III-1, x * 43000, about, amino acid sequence calculation, isozyme KAS III-2 Cuphea hookeriana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein] Nicotiana tabacum
-
 acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein] Brassica napus
-
 acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein] Cuphea hookeriana
-
 acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein] Spinacia oleracea
-
 acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 additional information Nicotiana tabacum fatty acid composition and acyl-ACP content in the leaves ?
-
 ?
2.3.1.180 additional information Spinacia oleracea fatty acid composition and acyl-ACP content in the leaves ?
-
 ?
2.3.1.180 additional information Brassica napus fatty acid composition and acyl-ACP content in the seeds ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.180 Brassica napus
-
-
-
2.3.1.180 Cuphea hookeriana
-
 two isozymes KAS III-1 and KAS III-2
-
2.3.1.180 Nicotiana tabacum
-
-
-
2.3.1.180 Spinacia oleracea Q07510
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.180 embryo
-
 Brassica napus
-
2.3.1.180 flower constitutive expression of isozymes KAS III-1 and KAS III-2 Cuphea hookeriana
-
2.3.1.180 leaf
-
 Nicotiana tabacum
-
2.3.1.180 leaf
-
 Spinacia oleracea
-
2.3.1.180 leaf constitutive expression of isozymes KAS III-1 and KAS III-2 Cuphea hookeriana
-
2.3.1.180 additional information quantitatiev RT-PCR expression analysis of isozymes KAS III-1 and KAS III-2 Cuphea hookeriana
-
2.3.1.180 root constitutive expression of isozymes KAS III-1 and KAS III-2 Cuphea hookeriana
-
2.3.1.180 seed
-
 Brassica napus
-
2.3.1.180 seed developing, constitutive expression of isozymes KAS III-1 and KAS III-2 Cuphea hookeriana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.180 additional information
-
 activity of wild-type embryo and those from transgenic plants Brassica napus
2.3.1.180 0.000106
-
 wild-type leaves Nicotiana tabacum
2.3.1.180 0.0062
-
 transgenic leaves transformed with the Cuphea glaucescens enzyme, average value Nicotiana tabacum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein]
-
 Nicotiana tabacum acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein]
-
 Brassica napus acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein]
-
 Cuphea hookeriana acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein]
-
 Spinacia oleracea acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?
2.3.1.180 additional information fatty acid composition and acyl-ACP content in the leaves Nicotiana tabacum ?
-
 ?
2.3.1.180 additional information fatty acid composition and acyl-ACP content in the leaves Spinacia oleracea ?
-
 ?
2.3.1.180 additional information fatty acid composition and acyl-ACP content in the seeds Brassica napus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.180 ? x * 42000, about, amino acid sequence calculation, isozyme KAS III-1, x * 43000, about, amino acid sequence calculation, isozyme KAS III-2 Cuphea hookeriana

Synonyms

EC Number Synonyms Comment Organism
2.3.1.180 3-ketoacyl-acyl-carrier protein synthase III
-
 Nicotiana tabacum
2.3.1.180 3-ketoacyl-acyl-carrier protein synthase III
-
 Brassica napus
2.3.1.180 3-ketoacyl-acyl-carrier protein synthase III
-
 Cuphea hookeriana
2.3.1.180 3-ketoacyl-acyl-carrier protein synthase III
-
 Spinacia oleracea
2.3.1.180 ACP synthase III
-
 Nicotiana tabacum
2.3.1.180 ACP synthase III
-
 Brassica napus
2.3.1.180 ACP synthase III
-
 Cuphea hookeriana
2.3.1.180 ACP synthase III
-
 Spinacia oleracea
2.3.1.180 KAS III
-
 Nicotiana tabacum
2.3.1.180 KAS III
-
 Brassica napus
2.3.1.180 KAS III
-
 Cuphea hookeriana
2.3.1.180 KAS III
-
 Spinacia oleracea

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.180 30
-
 assay at Nicotiana tabacum
2.3.1.180 30
-
 assay at Brassica napus
2.3.1.180 30
-
 assay at Cuphea hookeriana
2.3.1.180 30
-
 assay at Spinacia oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.180 6
-
 assay at Nicotiana tabacum
2.3.1.180 6
-
 assay at Brassica napus
2.3.1.180 6
-
 assay at Cuphea hookeriana
2.3.1.180 6
-
 assay at Spinacia oleracea

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.3.1.180 Cuphea hookeriana isozyme KAS III-2, amino acid sequence calculation
-
 5.9
2.3.1.180 Cuphea hookeriana isozyme KAS III-1, amino acid sequence calculation
-
 6.8