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Literature summary extracted from

  • Kanno, T.; Kasai, K.; Ikejiri-Kanno, Y.; Wakasa, K.; Tozawa, Y.
    In vitro reconstitution of rice anthranilate synthase: distinct functional properties of the alpha subunits OASA1 and OASA2 (2004), Plant Mol. Biol., 54, 11-23.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.3.27 isolation of beta-subunit genes OASB1 and OASB2 and synthesis of each anthranilate synthase alpha-subunit (IASA1 and OASA2) and beta-subunit (OASB1 and OASB2) in wheat germ cell-free system Oryza sativa

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.3.27 L-Trp feedback inhibition Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.27 0.00571
-
 chorismate alpha-subunit OASA1, cosubstrate: NH4+ Oryza sativa
4.1.3.27 0.00624
-
 chorismate alpha-subunit OASA1D (N323D), cosubstrate: NH4+ Oryza sativa
4.1.3.27 0.0634
-
 chorismate reconstituted enzyme OASA1-OASB1, cosubstrate: NH4+ Oryza sativa
4.1.3.27 0.0703
-
 chorismate reconstituted enzyme OASA1D(N323D)-OASB1, cosubstrate: NH4+ Oryza sativa
4.1.3.27 0.0828
-
 chorismate alpha-subunit OASA2, cosubstrate: NH4+ Oryza sativa
4.1.3.27 0.109
-
 chorismate reconstituted enzyme OASA1D(N323D)-OASB1, cosubstrate: L-Gln Oryza sativa
4.1.3.27 0.121
-
 chorismate reconstituted enzyme OASA1-OASB1, cosubstrate: L-Gln Oryza sativa
4.1.3.27 0.178
-
 chorismate reconstituted enzyme OASA2-OASB1, cosubstrate: L-Gln Oryza sativa
4.1.3.27 0.239
-
 chorismate reconstituted enzyme OASA2-OASB1, cosubstrate: NH4+ Oryza sativa

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.27 Oryza sativa
-
 cv. Nipponbare
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.27
-
 Oryza sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.27 chorismate + L-Gln
-
 Oryza sativa anthranilate + pyruvate + L-glutamate
-
 ?
4.1.3.27 chorismate + NH4+ alpha-subunits (OASA1 or OASA2) alone possess anthranilate synthase activity with NH4+ Oryza sativa anthranilate + pyruvate + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.1.3.27 OASA1 alpha-subunit of anthranilate synthase Oryza sativa
4.1.3.27 OASA2 alpha-subunit of anthranilate synthase Oryza sativa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.3.27 0.0061
-
 chorismate alpha-subunit OASA2, cosubstrate: NH4+ Oryza sativa
4.1.3.27 0.0124
-
 chorismate alpha-subunit OASA1D (N323D, cosubstrate: NH4+) Oryza sativa
4.1.3.27 0.0185
-
 chorismate alpha-subunit OASA1, cosubstrate: NH4+ Oryza sativa
4.1.3.27 0.079
-
 chorismate reconstituted enzyme OASA2-OASB1, cosubstrate: NH4+ Oryza sativa
4.1.3.27 0.228
-
 chorismate reconstituted enzyme OASA1D(N323D)-OASB1, cosubstrate: NH4+ Oryza sativa
4.1.3.27 0.24
-
 chorismate reconstituted enzyme OASA2-OASB1, cosubstrate: L-Gln Oryza sativa
4.1.3.27 0.27
-
 chorismate reconstituted enzyme OASA1-OASB1, cosubstrate: NH4+ Oryza sativa
4.1.3.27 0.48
-
 chorismate reconstituted enzyme OASA1D(N323D)-OASB1, cosubstrate: L-Gln Oryza sativa
4.1.3.27 0.58
-
 chorismate reconstituted enzyme OASA1-OASB1, cosubstrate: L-Gln Oryza sativa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.3.27 0.0053
-
 L-Trp reconstituted enzyme OASA2-OASB1, cosubstrate: L-Gln Oryza sativa
4.1.3.27 0.0397
-
 L-Trp reconstituted enzyme OASA1-OASB1, cosubstrate: L-Gln Oryza sativa
4.1.3.27 0.135
-
 L-Trp reconstituted enzyme OASA1D(N323D)-OASB1, cosubstrate: L-Gln Oryza sativa