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Literature summary extracted from
- Molecular cloning and characterization of Arabidopsis thaliana Golgi alpha-mannosidase II, a key enzyme in the formation of complex N-glycans in plants (2006), Plant J., 45, 789-803.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.114 | GMII, DNA and amino acid sequence determination and analysis, expression of His-tagged functional Golgi alpha-mannosidase II as soluble protein, which is secreted to the culture medium, in Spodoptera frugiperda Sf21 insect cells using the baculovirus transfection system, transient expression of GFP-tagged enzyme in Nicotiana benthamiana plants shows localization of the recombinant enzyme to the Golgi and endoplasmic reticulum | Arabidopsis thaliana |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.2.1.114 | additional information | construction of two Golgi alpha-mannosidase II knockout mutant plants, lines Salk_052443 and Salk_141821, resulting in predominant presence of unprocessed N-glycans but revealing also that alternative routes for N-glycan processing exist in the plants, mutant substrate specificity analysis, overview | Arabidopsis thaliana |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.114 | Cu2+ | over 97% inhibition of the recombinant GMII at 1 mM | Arabidopsis thaliana |  |
| 3.2.1.114 | additional information | no inhibition of recombinant GMII by EDTA | Arabidopsis thaliana | |
| 3.2.1.114 | swainsonine | 50% inhibition of recombinant GMII at 18 nM | Arabidopsis thaliana | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.114 | Golgi apparatus | Golgi alpha-mannosidase II, the enzyme has a transmembrane domain and a cytoplasmic tail sufficient for localisation of the enzyme to the Golgi complex, retention by lumenal sequences is not involved, retention mechanism | Arabidopsis thaliana | 5794 | - |
| 3.2.1.114 | membrane | Golgi alpha-mannosidase II is a type II membrane protein | Arabidopsis thaliana | 16020 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.114 | additional information | no effect on recombinant GMII through Ca2+, Co2+, Mn2+, Ni2+, and Zn2+ | Arabidopsis thaliana | |
| 3.2.1.114 | Zn2+ | the recombinant GMII contains zinc in a stoichiometric amount | Arabidopsis thaliana | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.114 | Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-1-Asn + H2O | Arabidopsis thaliana | physiological substrate, conversion by sequential removal of two alpha1,6-linked and alpha1,3-linked mannose residues from the alpha-1,6-branch of the substrate | Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-1-Asn + alpha-D-mannose | - |
? | |
| 3.2.1.114 | additional information | Arabidopsis thaliana | Golgi alpha-mannosidase II is a key enzyme in the formation of complex N-glycans in plants | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.114 | Arabidopsis thaliana | Q9LFR0 | - |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.114 | glycoprotein | recombinant secreted His-tagged soluble enzyme from culture medium of Spodoptera frugiperda Sf21 insect cells is N-glycosylated ans sensitive to PNGaseF | Arabidopsis thaliana |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.114 | recombinant secreted His-tagged soluble enzyme from culture medium of Spodoptera frugiperda Sf21 insect cells by nickel affinity chromatography | Arabidopsis thaliana |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.114 | 6.38 | - |
purified recombinant GMII expressed in insect cells, substrate 4-nitrophenyl alpha-mannoside | Arabidopsis thaliana |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.114 | 4-nitrophenyl alpha-D-mannoside + H2O | - |
Arabidopsis thaliana | 4-nitrophenol + alpha-D-mannose | - |
? | |
| 3.2.1.114 | Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))(Xylbeta(1-4))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-1-Asn + H2O | - |
Arabidopsis thaliana | Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))(Xylbeta(1-4))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-1-Asn + alpha-D-mannose | - |
? | |
| 3.2.1.114 | Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-1-Asn + H2O | physiological substrate, conversion by sequential removal of two alpha1,6-linked and alpha1,3-linked mannose residues from the alpha-1,6-branch of the substrate | Arabidopsis thaliana | Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-1-Asn + alpha-D-mannose | - |
? | |
| 3.2.1.114 | Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-1-Asn + H2O | conversion by sequential removal of two alpha1,6-linked and alpha1,3-linked mannose residues from the alpha-1,6-branch of the substrate | Arabidopsis thaliana | Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-1-Asn + alpha-D-mannose | - |
? | |
| 3.2.1.114 | Manalpha(1-6)(Manalpha(1-3))Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + H2O | - |
Arabidopsis thaliana | Manalpha(1-6)(GlcNAcbeta(1-2)Manalpha(1-3))Manbeta(1-4)GlcNAcbeta(1-4)GlcNAc-pyridylaminoside + alpha-D-mannose | - |
? | |
| 3.2.1.114 | additional information | substrate specificity, overview | Arabidopsis thaliana | ? | - |
? | |
| 3.2.1.114 | additional information | Golgi alpha-mannosidase II is a key enzyme in the formation of complex N-glycans in plants | Arabidopsis thaliana | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.114 | More | the enzyme has a transmembrane domain and a cytoplasmic tail sufficient for localisation of the enzyme to the Golgi complex, retention by lumenal sequences is not involved | Arabidopsis thaliana |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.114 | GmII | - |
Arabidopsis thaliana |
| 3.2.1.114 | Golgi alpha-mannosidase II | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.114 | 37 | - |
assay at | Arabidopsis thaliana |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.114 | 5.8 | - |
assay at | Arabidopsis thaliana |
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