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Literature summary extracted from

  • Ma, X.; Panjikar, S.; Koepke, J.; Loris, E.; Stockigt, J.
    The Structure of Rauvolfia serpentina Strictosidine Synthase Is a Novel Six-Bladed {beta}-Propeller Fold in Plant Proteins (2006), Plant Cell, 18, 907-920.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
4.3.3.2 medicine anti-arrhythmic heart disorders, ajmaline agents Rauvolfia serpentina
4.3.3.2 synthesis involved in the biosynthesis of almost all plant monoterpenoid indole alkaloids Rauvolfia serpentina

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.3.2 cloned with His-tag, expression in Escherichia coli strain M15, after expression and Ni-NTA column removed by DAPase Rauvolfia serpentina

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.3.3.2 crystals of free STR1 and co-crystallization of STR1 with each tryptamine and secologanin, crystal growing by hanging drop vapor diffusion, 302-304 K, 0.8 M potassium sodium tartrate tetrahydrate, pH 7.5 - 8.0, combined multiple wavelength anomalous dispersion and molecular replacement methods Rauvolfia serpentina

Protein Variants

EC Number Protein Variants Comment Organism
4.3.3.2 E309A mutant with 25% decrease of kcat Rauvolfia serpentina
4.3.3.2 H307A mutant with 130-fold increase of Km for secologanin Rauvolfia serpentina
4.3.3.2 Y151F mutant with 2.8-fold increase of Km for tryptamin Rauvolfia serpentina

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.3.3.2 2,4-dinitrofluorobenzene 5 mM, no inactivation after 30 min and 15% inactivation after 120 min in Tris-HCl, pH 8.0 Rauvolfia serpentina
4.3.3.2 4-(2-aminoethyl)-benzenesulfonyl fluoride 4 mM, 4% inactivation after 30 min and 12% inactivation after 120 min in 100 mM potassium phosphate buffer, pH 7.0 Rauvolfia serpentina
4.3.3.2 diethylpyrocarbonate 1 mM, 49% inactivation after 30 min and 63% inactivation after 120 min in 100 mM potassium phosphate, pH 6.0 Rauvolfia serpentina
4.3.3.2 additional information no inactivation by phenylmethanesulfonyl fluoride, L-chloro-3-(4-tosyl-amido)-4-phenyl-2-butanone and L-chloro-3-(4-tosyl-amido)-7-amino-2-heptanone after 30 and 120 min in 100 mM potassium phosphate buffer, pH 7.0 Rauvolfia serpentina
4.3.3.2 N,N'-dicyclohexylcarbodiimide 0.05 mM, complete inactivation after 30 min in MES, pH 6.0 Rauvolfia serpentina
4.3.3.2 N-Acetylimidazole 10 mM, 5% inactivation after 30 min and 12% inactivation after 120 min in Tris-HCl, pH 8.0 Rauvolfia serpentina
4.3.3.2 N-ethyl-5-phenylisoxazolium-3'-sulfonate 1 mM leads to 48% inactivation after 30 min and 53% inactivation after 120 min in MES, pH 6.0, 5 mM leads to 80% inactivation after 30 min and 100% inactivation after 120 min in MES, pH 6.0 Rauvolfia serpentina
4.3.3.2 p-chlormercuribenzoate 1 mM, 34% inactivation after 30 min and 35% inactivation after 120 min in 100 mM potassium phosphate, pH 6.0 Rauvolfia serpentina

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.3.2 0.0054
-
tryptamine E309A, pH 7.0, 35°C, 15 min Rauvolfia serpentina
4.3.3.2 0.0062
-
tryptamine wild type, pH 7.0, 35°C, 15 min Rauvolfia serpentina
4.3.3.2 0.0079
-
tryptamine H307A, pH 7.0, 35°C, 15 min Rauvolfia serpentina
4.3.3.2 0.0172
-
tryptamine Y151F, pH 7.0, 35°C, 15 min Rauvolfia serpentina
4.3.3.2 0.039
-
secologanin wild type, pH 7.0, 35°C, 15 min Rauvolfia serpentina
4.3.3.2 0.044
-
secologanin Y151F, pH 7.0, 35°C, 15 min Rauvolfia serpentina
4.3.3.2 0.095
-
secologanin E309A, pH 7.0, 35°C, 15 min Rauvolfia serpentina
4.3.3.2 5.07
-
secologanin H307A, pH 7.0, 35°C, 15 min Rauvolfia serpentina

Organism

EC Number Organism UniProt Comment Textmining
4.3.3.2 Rauvolfia serpentina P68175
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.3.2
-
Rauvolfia serpentina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.3.2 5,6-dihydroxytryptamine
-
Rauvolfia serpentina ?
-
?
4.3.3.2 5-fluorotryptamine
-
Rauvolfia serpentina ?
-
?
4.3.3.2 6-fluorotryptamine
-
Rauvolfia serpentina ?
-
?
4.3.3.2 6-methoxytryptamine
-
Rauvolfia serpentina ?
-
?
4.3.3.2 7-methyltryptamine
-
Rauvolfia serpentina ?
-
?
4.3.3.2 serotonin
-
Rauvolfia serpentina ?
-
?
4.3.3.2 tryptamine + secologanin
-
Rauvolfia serpentina 3alpha(S)-strictosidine
-
?

Synonyms

EC Number Synonyms Comment Organism
4.3.3.2 STR1
-
Rauvolfia serpentina