EC Number | Application | Comment | Organism |
---|---|---|---|
4.3.3.2 | medicine | anti-arrhythmic heart disorders, ajmaline agents | Rauvolfia serpentina |
4.3.3.2 | synthesis | involved in the biosynthesis of almost all plant monoterpenoid indole alkaloids | Rauvolfia serpentina |
EC Number | Cloned (Comment) | Organism |
---|---|---|
4.3.3.2 | cloned with His-tag, expression in Escherichia coli strain M15, after expression and Ni-NTA column removed by DAPase | Rauvolfia serpentina |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.3.3.2 | crystals of free STR1 and co-crystallization of STR1 with each tryptamine and secologanin, crystal growing by hanging drop vapor diffusion, 302-304 K, 0.8 M potassium sodium tartrate tetrahydrate, pH 7.5 - 8.0, combined multiple wavelength anomalous dispersion and molecular replacement methods | Rauvolfia serpentina |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.3.3.2 | E309A | mutant with 25% decrease of kcat | Rauvolfia serpentina |
4.3.3.2 | H307A | mutant with 130-fold increase of Km for secologanin | Rauvolfia serpentina |
4.3.3.2 | Y151F | mutant with 2.8-fold increase of Km for tryptamin | Rauvolfia serpentina |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.3.3.2 | 2,4-dinitrofluorobenzene | 5 mM, no inactivation after 30 min and 15% inactivation after 120 min in Tris-HCl, pH 8.0 | Rauvolfia serpentina | |
4.3.3.2 | 4-(2-aminoethyl)-benzenesulfonyl fluoride | 4 mM, 4% inactivation after 30 min and 12% inactivation after 120 min in 100 mM potassium phosphate buffer, pH 7.0 | Rauvolfia serpentina | |
4.3.3.2 | diethylpyrocarbonate | 1 mM, 49% inactivation after 30 min and 63% inactivation after 120 min in 100 mM potassium phosphate, pH 6.0 | Rauvolfia serpentina | |
4.3.3.2 | additional information | no inactivation by phenylmethanesulfonyl fluoride, L-chloro-3-(4-tosyl-amido)-4-phenyl-2-butanone and L-chloro-3-(4-tosyl-amido)-7-amino-2-heptanone after 30 and 120 min in 100 mM potassium phosphate buffer, pH 7.0 | Rauvolfia serpentina | |
4.3.3.2 | N,N'-dicyclohexylcarbodiimide | 0.05 mM, complete inactivation after 30 min in MES, pH 6.0 | Rauvolfia serpentina | |
4.3.3.2 | N-Acetylimidazole | 10 mM, 5% inactivation after 30 min and 12% inactivation after 120 min in Tris-HCl, pH 8.0 | Rauvolfia serpentina | |
4.3.3.2 | N-ethyl-5-phenylisoxazolium-3'-sulfonate | 1 mM leads to 48% inactivation after 30 min and 53% inactivation after 120 min in MES, pH 6.0, 5 mM leads to 80% inactivation after 30 min and 100% inactivation after 120 min in MES, pH 6.0 | Rauvolfia serpentina | |
4.3.3.2 | p-chlormercuribenzoate | 1 mM, 34% inactivation after 30 min and 35% inactivation after 120 min in 100 mM potassium phosphate, pH 6.0 | Rauvolfia serpentina |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.3.3.2 | 0.0054 | - |
tryptamine | E309A, pH 7.0, 35°C, 15 min | Rauvolfia serpentina | |
4.3.3.2 | 0.0062 | - |
tryptamine | wild type, pH 7.0, 35°C, 15 min | Rauvolfia serpentina | |
4.3.3.2 | 0.0079 | - |
tryptamine | H307A, pH 7.0, 35°C, 15 min | Rauvolfia serpentina | |
4.3.3.2 | 0.0172 | - |
tryptamine | Y151F, pH 7.0, 35°C, 15 min | Rauvolfia serpentina | |
4.3.3.2 | 0.039 | - |
secologanin | wild type, pH 7.0, 35°C, 15 min | Rauvolfia serpentina | |
4.3.3.2 | 0.044 | - |
secologanin | Y151F, pH 7.0, 35°C, 15 min | Rauvolfia serpentina | |
4.3.3.2 | 0.095 | - |
secologanin | E309A, pH 7.0, 35°C, 15 min | Rauvolfia serpentina | |
4.3.3.2 | 5.07 | - |
secologanin | H307A, pH 7.0, 35°C, 15 min | Rauvolfia serpentina |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.3.3.2 | Rauvolfia serpentina | P68175 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.3.3.2 | - |
Rauvolfia serpentina |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.3.3.2 | 5,6-dihydroxytryptamine | - |
Rauvolfia serpentina | ? | - |
? | |
4.3.3.2 | 5-fluorotryptamine | - |
Rauvolfia serpentina | ? | - |
? | |
4.3.3.2 | 6-fluorotryptamine | - |
Rauvolfia serpentina | ? | - |
? | |
4.3.3.2 | 6-methoxytryptamine | - |
Rauvolfia serpentina | ? | - |
? | |
4.3.3.2 | 7-methyltryptamine | - |
Rauvolfia serpentina | ? | - |
? | |
4.3.3.2 | serotonin | - |
Rauvolfia serpentina | ? | - |
? | |
4.3.3.2 | tryptamine + secologanin | - |
Rauvolfia serpentina | 3alpha(S)-strictosidine | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.3.3.2 | STR1 | - |
Rauvolfia serpentina |