BRENDA - Enzyme Database

Molecular cloning and functional expression of enolase from Trichinella spiralis

Nakada, T.; Nagano, I.; Wu, Z.; Takahashi, Y.; Parasitol. Res. 96, 354-360 (2005)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.11
expression in Escherichia coli strain DH5alpha with His-tag, recombinant enzyme without enolase activity, gaines activity after cutting off the signal peptide from the full-length protein
Trichinella spiralis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
46000
-
recombinant enzyme, Western blotting
Trichinella spiralis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Trichinella spiralis
-
phosphoenolpyruvate + H2O
-
-
r
4.2.1.11
2-phospho-D-glycerate
Trichinella spiralis ISS413
-
phosphoenolpyruvate + H2O
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Trichinella spiralis
-
-
-
4.2.1.11
Trichinella spiralis ISS413
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
muscle-stage larvae isolated by pepsin-HCl digestion from mice, recombinant enzyme by His Trap chelating column
Trichinella spiralis
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
muscle
-
Trichinella spiralis
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
666529
Trichinella spiralis
phosphoenolpyruvate + H2O
-
-
-
r
4.2.1.11
2-phospho-D-glycerate
-
666529
Trichinella spiralis ISS413
phosphoenolpyruvate + H2O
-
-
-
r
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
6.8
-
-
Trichinella spiralis
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.2.1.11
6
7.5
-
Trichinella spiralis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
expression in Escherichia coli strain DH5alpha with His-tag, recombinant enzyme without enolase activity, gaines activity after cutting off the signal peptide from the full-length protein
Trichinella spiralis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
46000
-
recombinant enzyme, Western blotting
Trichinella spiralis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Trichinella spiralis
-
phosphoenolpyruvate + H2O
-
-
r
4.2.1.11
2-phospho-D-glycerate
Trichinella spiralis ISS413
-
phosphoenolpyruvate + H2O
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
muscle-stage larvae isolated by pepsin-HCl digestion from mice, recombinant enzyme by His Trap chelating column
Trichinella spiralis
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
muscle
-
Trichinella spiralis
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
666529
Trichinella spiralis
phosphoenolpyruvate + H2O
-
-
-
r
4.2.1.11
2-phospho-D-glycerate
-
666529
Trichinella spiralis ISS413
phosphoenolpyruvate + H2O
-
-
-
r
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.2.1.11
6.8
-
-
Trichinella spiralis
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
4.2.1.11
6
7.5
-
Trichinella spiralis