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Literature summary extracted from

  • Yue, J.; Xiong, W.; Ferrell, J.E.
    B-Raf and C-Raf are required for Ras-stimulated p42 MAP kinase activation in Xenopus egg extracts (2006), Oncogene, 1, 1-9.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.25 Ras activates C-Raf and B-Raf Xenopus laevis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.11.25 B-RAf, DNA and amino acid sequence determination and analysis, expression of His6-tagged B-Raf in bacteria, expression of His6-tagged and FLAG-tagged C-Raf in insect cells Xenopus laevis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.25 Golgi apparatus activation of MEK1 by C-Raf Xenopus laevis 5794
-
2.7.11.25 kinetochore activation of p42 MAPK Xenopus laevis 776
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.25 Mg2+
-
 Xenopus laevis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.25 ATP + MEK1 Xenopus laevis Ras-induced activation of the MAPK signaling cascade ADP + phosphorylated MEK1
-
 ?
2.7.11.25 ATP + p42 MAPK Xenopus laevis Ras-induced activation of the MAPK signaling cascade ADP + phosphorylated p42 MAPK
-
 ?
2.7.11.25 additional information Xenopus laevis B-Raf and C-Raf, but not Mos, are required for Ras-induced MEK1 and p42 MAPK activation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.25 Xenopus laevis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.25 partial purification of Mos and B-Raf from egg extracts by heparin affinity chromatography and/or ion exchange chromatography, and GST-MEK1 affinity chromatography, recombinant His6-tagged B-Raf by nickel affinity chromatography Xenopus laevis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.25 egg no changes in B-Raf activity after fertilization Xenopus laevis
-
2.7.11.25 embryo no changes in B-Raf activity in the first embryonic cell cycles Xenopus laevis
-
2.7.11.25 oocyte no changes in B-Raf activity during progesterone-induced maturation Xenopus laevis
-
2.7.11.25 zygote
-
 Xenopus laevis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.25 ATP + histone H1
-
 Xenopus laevis ADP + phosphorylated histone H1
-
 ?
2.7.11.25 ATP + MEK1 Ras-induced activation of the MAPK signaling cascade Xenopus laevis ADP + phosphorylated MEK1
-
 ?
2.7.11.25 ATP + MEK1 the substrate of B-Raf and C-Raf is a MAPKK, no substrate of Mos, recombinant GST-MEK1 substrate Xenopus laevis ADP + phosphorylated MEK1
-
 ?
2.7.11.25 ATP + p42 MAPK Ras-induced activation of the MAPK signaling cascade Xenopus laevis ADP + phosphorylated p42 MAPK
-
 ?
2.7.11.25 ATP + p42 MAPK the substrate of B-Raf and C-Raf, no substrate of Mos, recombinant His6-p42 MAPK substrate Xenopus laevis ADP + phosphorylated p42 MAPK
-
 ?
2.7.11.25 additional information B-Raf and C-Raf, but not Mos, are required for Ras-induced MEK1 and p42 MAPK activation Xenopus laevis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.25 B-Raf
-
 Xenopus laevis
2.7.11.25 C-Raf
-
 Xenopus laevis
2.7.11.25 MAP kinase kinase kinase
-
 Xenopus laevis
2.7.11.25 MAPKKK
-
 Xenopus laevis
2.7.11.25 Mos
-
 Xenopus laevis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.25 30
-
 assay at Xenopus laevis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.25 7.3
-
 assay at Xenopus laevis

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.25 ATP
-
 Xenopus laevis