Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Dong, Y.H.; Wang, L.H.; Xu, J.L.; Zhang, H.B.; Zhang, X.F.; Zhang, L.H.
    Quenching quorum-sensing-dependent bacterial infection by an N-acyl homoserine lactonase (2001), Nature, 411, 813-817.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.81 expression of GST-tagged AiiA containing a thrombin cleavage site in Escherichia coli strain DH5alpha, construction of transgenic Solanum tuberosum and Nicotiana tabacum plants expressing gene aiiA Bacillus sp. (in: Bacteria)

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.81 additional information construction of transgenic Solanum tuberosum and Nicotiana tabacum plants via Agrobacterium tumefaciens transfection with Bacillus sp. gene aiiA inserted into their genome, the transgenic plants express the recombinant aiiA gene and show increased AHL-lactonase activity compared to the wild-type plants, as well as enhanced resistance to plant pathogen Erwinia carotovora, the recombinant enzyme might be trapped in microsomes Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.81 N-acyl-(S)-homoserine lactone + H2O Bacillus sp. (in: Bacteria) the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, acylhomoserine lactones are autoinducers of quorum-sensing signaling, the inhibition of which is a feasible approach for prevention of bacterial infection N-acyl-(S)-homoserine
-
 ?
3.1.1.81 N-acyl-(S)-homoserine lactone + H2O Bacillus sp. (in: Bacteria) 240B1 the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, acylhomoserine lactones are autoinducers of quorum-sensing signaling, the inhibition of which is a feasible approach for prevention of bacterial infection N-acyl-(S)-homoserine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.81 Bacillus sp. (in: Bacteria) Q9L8R8 isolated from soil, gene aiiA
-
3.1.1.81 Bacillus sp. (in: Bacteria) 240B1 Q9L8R8 isolated from soil, gene aiiA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.81 recombinant GST-tagged AiiA from Escherichia coli strain DH5alpha by glutathione affinity chromatography, the GST-atg is cleaved off by thrombin Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.81 additional information the substrate N-acyl-L-homoserine lactones show negligible enzyme-independent delactonization Bacillus sp. (in: Bacteria) ?
-
 ?
3.1.1.81 additional information the substrate N-acyl-L-homoserine lactones show negligible enzyme-independent delactonization Bacillus sp. (in: Bacteria) 240B1 ?
-
 ?
3.1.1.81 N-3-oxododecanoyl-L-homoserine lactone + H2O the substrate regulates production of virulence determinants of the human pathogen Pseudomonas aeruginosa Bacillus sp. (in: Bacteria) N-3-oxododecanoyl-L-homoserine product identification by mass spectrometry ?
3.1.1.81 N-3-oxododecanoyl-L-homoserine lactone + H2O the substrate regulates production of virulence determinants of the human pathogen Pseudomonas aeruginosa Bacillus sp. (in: Bacteria) 240B1 N-3-oxododecanoyl-L-homoserine product identification by mass spectrometry ?
3.1.1.81 N-3-oxohexanoyl-L-homoserine lactone + H2O
-
 Bacillus sp. (in: Bacteria) N-3-oxohexanoyl-L-homoserine product identification by mass spectrometry ?
3.1.1.81 N-3-oxohexanoyl-L-homoserine lactone + H2O
-
 Bacillus sp. (in: Bacteria) 240B1 N-3-oxohexanoyl-L-homoserine product identification by mass spectrometry ?
3.1.1.81 N-3-oxooctanoyl-L-homoserine lactone + H2O the substrate controls Ti plasmid conjugal transfer in Agrobacterium tumefaciens Bacillus sp. (in: Bacteria) N-3-oxooctanoyl-L-homoserine product identification by mass spectrometry ?
3.1.1.81 N-3-oxooctanoyl-L-homoserine lactone + H2O the substrate controls Ti plasmid conjugal transfer in Agrobacterium tumefaciens Bacillus sp. (in: Bacteria) 240B1 N-3-oxooctanoyl-L-homoserine product identification by mass spectrometry ?
3.1.1.81 N-acyl-(S)-homoserine lactone + H2O the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, acylhomoserine lactones are autoinducers of quorum-sensing signaling, the inhibition of which is a feasible approach for prevention of bacterial infection Bacillus sp. (in: Bacteria) N-acyl-(S)-homoserine
-
 ?
3.1.1.81 N-acyl-(S)-homoserine lactone + H2O the enzyme AiiA inactivates the acylhomoserine lactone quorum-sensing signal and attenuates the virulence of Erwinia carotovora, acylhomoserine lactones are autoinducers of quorum-sensing signaling, the inhibition of which is a feasible approach for prevention of bacterial infection Bacillus sp. (in: Bacteria) 240B1 N-acyl-(S)-homoserine
-
 ?
3.1.1.81 N-butanoyl-L-homoserine lactone + H2O the substrate regulates production of virulence determinants of the human pathogen Pseudomonas aeruginosa Bacillus sp. (in: Bacteria) N-butanoyl-L-homoserine product identification by mass spectrometry ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.81 AHL-lactonase
-
 Bacillus sp. (in: Bacteria)
3.1.1.81 delactonase
-
 Bacillus sp. (in: Bacteria)
3.1.1.81 N-acyl homoserine lactonase
-
 Bacillus sp. (in: Bacteria)
3.1.1.81 quorum-quenching enzyme
-
 Bacillus sp. (in: Bacteria)