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Literature summary extracted from

  • Kuhn, D.; Wiese, M.
    LmxPK4, a mitogen-activated protein kinase kinase homologue of Leishmania mexicana with a potential role in parasite differentiation (2005), Mol. Microbiol., 56, 1169-1182.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.12.2 DNA and amino acid sequence determination and analysis, expression of wild-type and mutant GST-fusion enzymes in Escherichia coli, expression of a GFP-tagged enzyme in the null mutant strain, complementation of the null mutant strain by expression of the enzyme from plasmid or by reintegration of the wild-type gene into the genomic DNA locus Leishmania mexicana

Protein Variants

EC Number Protein Variants Comment Organism
2.7.12.2 K102R site-directed mutagenesis, kinase inactive mutant, but slight autophosphorylation activity, the mutant strain shows a phenotype with a proliferation defect after infection of human macrophages and no or delayed lesion development in mice Leishmania mexicana
2.7.12.2 additional information construction of deletion/disruption, null, or loss-of-function mutants, which all show a phenotype with a proliferation defect after infection of human macrophages and no or delayed lesion development in mice, gene replacement method Leishmania mexicana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.12.2 cytosol
-
 Leishmania mexicana 5829
-
2.7.12.2 flagellum of promastigotes Leishmania mexicana
-
-
2.7.12.2 nucleus
-
 Leishmania mexicana 5634
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.12.2 38900
-
 x * 38900, sequence calculation, x * 39000, recombinant enzyme, SDS-PAGE Leishmania mexicana
2.7.12.2 39000
-
 x * 38900, sequence calculation, x * 39000, recombinant enzyme, SDS-PAGE Leishmania mexicana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.12.2 ATP + MAP kinase Leishmania mexicana the enzyme is involved in the mitogen-activated protein kinase signaling pathway important for e.g. flagellar length control, the enzyme is involved in differentiation of the parasite and is important for virulence and infection of human peritoneal macrophages ADP + phosphorylated MAP kinase
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.12.2 Leishmania mexicana Q9GRT1
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.12.2 phosphoprotein the enzyme performs autophosphorylation Leishmania mexicana

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.12.2 recombinant wild-type and mutant GST-fusion enzymes from Escherichia coli by glutathione affinity chromatography Leishmania mexicana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.12.2 additional information the enzyme is developmentally regulated expressed, no expression in the amastigote stage in human host cells Leishmania mexicana
-
2.7.12.2 promastigote and during differentiation to amastigote Leishmania mexicana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.12.2 ATP + MAP kinase the enzyme is involved in the mitogen-activated protein kinase signaling pathway important for e.g. flagellar length control, the enzyme is involved in differentiation of the parasite and is important for virulence and infection of human peritoneal macrophages Leishmania mexicana ADP + phosphorylated MAP kinase
-
 ?
2.7.12.2 ATP + MAP kinase docking site for the substrate is located close to the N-terminal Lys4 and consists of 2 basic amino acid residues separated by a spacer of 2-6 variable residues from an L/I/V-X-L/I/V sequence Leishmania mexicana ADP + phosphorylated MAP kinase
-
 ?
2.7.12.2 ATP + myelin basic protein
-
 Leishmania mexicana ADP + phosphorylated myelin basic protein
-
 ?
2.7.12.2 additional information the enzyme performs autophosphorylation Leishmania mexicana ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.12.2 ? x * 38900, sequence calculation, x * 39000, recombinant enzyme, SDS-PAGE Leishmania mexicana
2.7.12.2 More the enzyme contains a protein kinase domain, residues L73-I331, with 11 highly conserved subdomains, and a subdomain II with an invariable lysine residue responsible for ATP binding Leishmania mexicana

Synonyms

EC Number Synonyms Comment Organism
2.7.12.2 MAP kinase kinase homologue
-
 Leishmania mexicana
2.7.12.2 mitogen-activated protein kinase kinase 4
-
 Leishmania mexicana
2.7.12.2 mitogen-activated protein kinase kinase homologue
-
 Leishmania mexicana
2.7.12.2 MKK homologue
-
 Leishmania mexicana
2.7.12.2 PK4
-
 Leishmania mexicana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.12.2 40
-
-
 Leishmania mexicana

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.12.2 ATP subdomain II with an invariable lysine residue is responsible for ATP binding Leishmania mexicana

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.7.12.2 Leishmania mexicana sequence calculation
-
 7.4