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Literature summary extracted from

  • Gould, T.A.; Schweizer, H.P.; Churchill, M.E.
    Structure of the Pseudomonas aeruginosa acyl-homoserinelactone synthase LasI (2004), Mol. Microbiol., 53, 1135-1146.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.184 functional overexpression of His-tagged LasIDELTAG and point mutants in Escherichia coli as soluble proteins Pseudomonas aeruginosa

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.184 purified recombinant detagged LasIDELTAG, hanging drop vapour diffusion method, from 1.5 M ammonium sulfate, 0.125 M disodium sulfate, and 0.1 M MOPS, pH 6.5, cryoprotection with 15% glycerol, heavy atom derivatization with Hg2+, single isomorphous replacement with anomalous scattering, X-ray diffraction structure determination and analysis at 2.3-3.1 A resolution, structure modeling Pseudomonas aeruginosa

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.184 K150E/R154QE site-directed mutagenesis, the mutant shows highly reduced activity compared to the fully active mutant LasIDELTAG Pseudomonas aeruginosa
2.3.1.184 K150Q site-directed mutagenesis, the mutant shows similar activity as the fully active mutant LasIDELTAG Pseudomonas aeruginosa
2.3.1.184 K150Q/R154Q site-directed mutagenesis, the mutant shows reduced activity compared to the fully active mutant LasIDELTAG Pseudomonas aeruginosa
2.3.1.184 R154E site-directed mutagenesis, the mutant shows reduced activity compared to the fully active mutant LasIDELTAG Pseudomonas aeruginosa
2.3.1.184 R154Q site-directed mutagenesis, the mutant shows similar activity as the fully active mutant LasIDELTAG Pseudomonas aeruginosa
2.3.1.184 R172A site-directed mutagenesis, the mutant shows increased activity compared to the fully active mutant LasIDELTAG Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.184 additional information Pseudomonas aeruginosa LasI and LasR form a quorum-sensing system playing a pivotal role in virulence gene regulation of the opportunistic human pathogen Pseudomonas aeruginosa ?
-
 ?
2.3.1.184 S-adenosyl-L-methionine + 3-oxododecanoyl-[acyl-carrier protein] Pseudomonas aeruginosa substrate of LasI 5'-methylthioadenosine + N-3-oxododecanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.184 Pseudomonas aeruginosa
-
 gene lasI
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.184 recombinant His-tagged LasIDELTAG from Escherichia coli by nickel affinity chromatography and gel filtration, the His-tag is removed by thrombin cleavage Pseudomonas aeruginosa

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.184 an acyl-[acyl-carrier protein] + S-adenosyl-L-methionine = an [acyl-carrier protein] + S-methyl-5'-thioadenosine + an N-acyl-L-homoserine lactone substrate binding structure Pseudomonas aeruginosa
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.184 additional information
-
 activities of mutant LasI enzymes Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.184 additional information LasI and LasR form a quorum-sensing system playing a pivotal role in virulence gene regulation of the opportunistic human pathogen Pseudomonas aeruginosa Pseudomonas aeruginosa ?
-
 ?
2.3.1.184 S-adenosyl-L-methionine + 3-oxododecanoyl-[acyl-carrier protein] substrate of LasI Pseudomonas aeruginosa 5'-methylthioadenosine + N-3-oxododecanoyl-L-homoserine-1,4-lactone + [acyl-carrier protein]
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.184 acyl-homoserinelactone synthase
-
 Pseudomonas aeruginosa
2.3.1.184 AHL synthase
-
 Pseudomonas aeruginosa
2.3.1.184 LasI
-
 Pseudomonas aeruginosa
2.3.1.184 LasR
-
 Pseudomonas aeruginosa