Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Carlson, K.; Lagerback, P.; Nystrom, A.C.
    Bacteriophage T4 endonuclease II: concerted single-strand nicks yield double-strand cleavage (2004), Mol. Microbiol., 52, 1403-1411.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.21.2 Mg2+ absolutely required for nicking activity Tequatrovirus T4

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.21.2 double-stranded alkylated DNA + H2O Tequatrovirus T4 in vivo the enzyme produces mostly double-stranded fragments with a few internal nicks 5'-phosphooligonucleotides
-
ir

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.2 Tequatrovirus T4
-
Escherichia coli phage, i.e. Coliphage T4
-
3.1.21.8 Tequatrovirus T4 P07059
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.21.2 Endonucleolytic cleavage of ssDNA at apurinic/apyrimidinic sites to 5'-phosphooligonucleotide end-products reaction mechanism modelling Tequatrovirus T4

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.2 double-stranded alkylated DNA + H2O in vivo the enzyme produces mostly double-stranded fragments with a few internal nicks Tequatrovirus T4 5'-phosphooligonucleotides
-
ir
3.1.21.2 double-stranded alkylated DNA + H2O the enzyme cleaves sites of DNA with conserved sequence elements to both the left and the right of the cleaved bonds comprising 16 basepairs with some variability tolerated, nicking of a single and both strands, the latter in two nicking events, substrate specificity, overview, in vitro the enzyme produces mostly single-stranded nicks Tequatrovirus T4 5'-phosphooligonucleotides
-
ir
3.1.21.8 additional information EndoII nicks both strands simultaneously at an in vivo-favoured site but not at an in vitro-favoured site. The right-side conserved sequence element at in vivo-favoured sites is important for simultaneous nicking of both strands, generating double-strand cleavage. EndoII nicks the lower strand about 1.5fold faster than the upper strand. In addition, the upper and lower strands are nicked independently of each other, seldom resulting in double-strand cleavage Tequatrovirus T4 ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.1.21.2 EndoII
-
Tequatrovirus T4
3.1.21.2 endonuclease II
-
Tequatrovirus T4

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.21.2 30
-
assay at Tequatrovirus T4

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.21.2 8
-
assay at Tequatrovirus T4