EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.1.21.2 | Mg2+ | absolutely required for nicking activity | Tequatrovirus T4 |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.21.2 | double-stranded alkylated DNA + H2O | Tequatrovirus T4 | in vivo the enzyme produces mostly double-stranded fragments with a few internal nicks | 5'-phosphooligonucleotides | - |
ir |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.21.2 | Tequatrovirus T4 | - |
Escherichia coli phage, i.e. Coliphage T4 | - |
3.1.21.8 | Tequatrovirus T4 | P07059 | - |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.1.21.2 | Endonucleolytic cleavage of ssDNA at apurinic/apyrimidinic sites to 5'-phosphooligonucleotide end-products | reaction mechanism modelling | Tequatrovirus T4 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.21.2 | double-stranded alkylated DNA + H2O | in vivo the enzyme produces mostly double-stranded fragments with a few internal nicks | Tequatrovirus T4 | 5'-phosphooligonucleotides | - |
ir | |
3.1.21.2 | double-stranded alkylated DNA + H2O | the enzyme cleaves sites of DNA with conserved sequence elements to both the left and the right of the cleaved bonds comprising 16 basepairs with some variability tolerated, nicking of a single and both strands, the latter in two nicking events, substrate specificity, overview, in vitro the enzyme produces mostly single-stranded nicks | Tequatrovirus T4 | 5'-phosphooligonucleotides | - |
ir | |
3.1.21.8 | additional information | EndoII nicks both strands simultaneously at an in vivo-favoured site but not at an in vitro-favoured site. The right-side conserved sequence element at in vivo-favoured sites is important for simultaneous nicking of both strands, generating double-strand cleavage. EndoII nicks the lower strand about 1.5fold faster than the upper strand. In addition, the upper and lower strands are nicked independently of each other, seldom resulting in double-strand cleavage | Tequatrovirus T4 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.21.2 | EndoII | - |
Tequatrovirus T4 |
3.1.21.2 | endonuclease II | - |
Tequatrovirus T4 |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.21.2 | 30 | - |
assay at | Tequatrovirus T4 |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.21.2 | 8 | - |
assay at | Tequatrovirus T4 |