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Literature summary extracted from

  • Lin, Y.H.; Xu, J.L.; Hu, J.; Wang, L.H.; Ong, S.L.; Leadbetter, J.R.; Zhang, L.H.
    Acyl-homoserine lactone acylase from Ralstonia strain XJ12B represents a novel and potent class of quorum-quenching enzymes (2003), Mol. Microbiol., 47, 849-860.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.5.1.97 biotechnology the enzyme can be used in quenching quorum sensing Ralstonia sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.97 gene aiiD, DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli strain DH5alpha, expression of AiiD in Pseudomonas aeruginosa strain PAO1 without showing catalytic function, but reducing the ability of the bacterium to exhibit quorum-regulated swarming motility, the bacterium is attenuated in nematode killing Ralstonia sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.81 additional information Ralstonia sp. the enzyme is involved in inactivation of N-acylhomoserine lactone signaling in regulation of infection and virulence functions ?
-
 ?
3.5.1.97 N-acyl-(S)-homoserine lactone + H2O Ralstonia sp. inactivation of N-acylhomoserine lactone signaling in regulation of infection and virulence functions (S)-homoserine lactone + carboxylic acid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.81 Ralstonia sp.
-
 isolated from a mixed-species biofilm
-
3.5.1.97 Ralstonia sp.
-
 gene aiiD, isolated from a mixed-species biofilm
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.81 additional information the enzyme is involved in inactivation of N-acylhomoserine lactone signaling in regulation of infection and virulence functions Ralstonia sp. ?
-
 ?
3.5.1.97 additional information no activity with penicillin Ralstonia sp. ?
-
 ?
3.5.1.97 N-3-oxodecanoyl-(S)-homoserine lactone + H2O hydrolysis of the amide bond Ralstonia sp. (S)-homoserine lactone + 3-oxodecanoic acid
-
 ?
3.5.1.97 N-acyl-(S)-homoserine lactone + H2O inactivation of N-acylhomoserine lactone signaling in regulation of infection and virulence functions Ralstonia sp. (S)-homoserine lactone + carboxylic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.81 AHL lactonase
-
 Ralstonia sp.
3.1.1.81 AHL-lactonase
-
 Ralstonia sp.
3.1.1.81 More the enzyme belongs to the quorum-quenching enzymes Ralstonia sp.
3.1.1.81 N-acyl-homoserine lactonase
-
 Ralstonia sp.
3.1.1.81 N-acyl-homoserine lactone lactonase
-
 Ralstonia sp.
3.1.1.81 quorum-quenching enzyme
-
 Ralstonia sp.
3.5.1.97 acyl-homoserine lactone acylase
-
 Ralstonia sp.
3.5.1.97 AHL-acylase
-
 Ralstonia sp.
3.5.1.97 AiiD
-
 Ralstonia sp.
3.5.1.97 More the enzyme belongs to the quorum-quenching enzymes Ralstonia sp.
3.5.1.97 quorum-quenching enzyme
-
 Ralstonia sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.97 30
-
 assay at Ralstonia sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.97 7
-
 assay at Ralstonia sp.