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Literature summary extracted from

  • Timm, J.; Van Rompaey, I.; Tricot, C.; Massaer, M.; Haeseleer, F.; Fauconnier, A.; Stalon, V.; Bollen, A.; Jacobs, P.
    Molecular cloning, characterization and purification of ornithine carbamoyltransferase from Mycobacterium bovis BCG (1992), Mol. Gen. Genet., 234, 475-480.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.3.3 expression in Escherichia coli Mycobacterium tuberculosis variant bovis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.3.3 arginine
-
 Mycobacterium tuberculosis variant bovis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.3.3 0.625
-
 Carbamoyl phosphate pH 8.5, 10 mM fixed substrate Mycobacterium tuberculosis variant bovis
2.1.3.3 0.85
-
 ornithine pH 8.5, 10 mM fixed substrate Mycobacterium tuberculosis variant bovis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.3.3 38000
-
 3 * 38000, PAGE Mycobacterium tuberculosis variant bovis
2.1.3.3 110000
-
 gel filtration Mycobacterium tuberculosis variant bovis

Organism

EC Number Organism UniProt Comment Textmining
2.1.3.3 Mycobacterium tuberculosis variant bovis
-
 BCG cells
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.3.3
-
 Mycobacterium tuberculosis variant bovis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.3.3 carbamoyl phosphate + L-ornithine
-
 Mycobacterium tuberculosis variant bovis phosphate + citrulline
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.3.3 trimer 3 * 38000, PAGE Mycobacterium tuberculosis variant bovis

Synonyms

EC Number Synonyms Comment Organism
2.1.3.3 otcase
-
 Mycobacterium tuberculosis variant bovis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.3.3 8.5
-
 in 50 mM EDTA/NaOH buffer Mycobacterium tuberculosis variant bovis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.1.3.3 8.2
-
 arginine determined in the presence of 0.5 mM ornithine and 0.5 mM carbamoylphosphate Mycobacterium tuberculosis variant bovis