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Literature summary extracted from

  • Garrido, M.N.; Lisa, T.A.; Domenech, C.E.
    Pseudomonas aeruginosa acid phosphatase contains an anionic site with a trimethyl subsite. Kinetic evidence obtained with alkylammonium ions (1988), Mol. Cell. Biochem., 84, 41-49.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.2 2-amino-2-hydroxymethyl-1,3-propanediol
-
 Pseudomonas aeruginosa
3.1.3.2 2-amino-2-methyl-1,3-propanediol
-
 Pseudomonas aeruginosa
3.1.3.2 2-amino-2-methyl-1-propanol
-
 Pseudomonas aeruginosa
3.1.3.2 alaninol
-
 Pseudomonas aeruginosa
3.1.3.2 additional information no or poor inhibition by DL-serine and D-alanine, and by 1-propanol, 3-methyl-1-butanol, 3-methyl-1-butanol, and choline, enzyme inhibition is more effective with N-trimethylated compounds than with N-triethylated ones Pseudomonas aeruginosa
3.1.3.2 N-tetraethylammonium
-
 Pseudomonas aeruginosa
3.1.3.2 N-tetramethylammonium
-
 Pseudomonas aeruginosa
3.1.3.2 N-trimethylammonium
-
 Pseudomonas aeruginosa
3.1.3.2 serinol
-
 Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.2 additional information
-
 additional information kinetics Pseudomonas aeruginosa
3.1.3.2 0.35
-
 Mg2+ pH 5.0, 37°C, partially purified enzyme Pseudomonas aeruginosa
3.1.3.2 2
-
 4-nitrophenyl phosphate pH 5.0, 37°C, partially purified enzyme Pseudomonas aeruginosa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.3.2 periplasm
-
 Pseudomonas aeruginosa
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.2 Mg2+ activates Pseudomonas aeruginosa
3.1.3.2 additional information no effect by NH4Cl, LiCl, and KCl Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.2 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.2 partially by preparation of periplasmic fraction including MgCl2 treatment and ammonium sulfate precipitation, co-purification of cholinesterase, which is removed by gel filtration Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.2 1
-
 partially purified enzyme Pseudomonas aeruginosa

Storage Stability

EC Number Storage Stability Organism
3.1.3.2 4°C, partially purified enzyme, precipitated by 90% ammonium sulfate, in Tris-HCl, pH 7.4, stable for at least 3 months Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.2 4-nitrophenyl phosphate + H2O
-
 Pseudomonas aeruginosa 4-nitrophenol + phosphate
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.2 37
-
 assay at Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.2 5
-
 assay at Pseudomonas aeruginosa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.1.3.2 additional information
-
 additional information inhibition kinetics Pseudomonas aeruginosa
3.1.3.2 0.6
-
 N-tetramethylammonium pH 5.0, 37°C, partially purified enzyme Pseudomonas aeruginosa
3.1.3.2 4.2
-
 N-tetraethylammonium pH 5.0, 37°C, partially purified enzyme Pseudomonas aeruginosa