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Literature summary extracted from

  • Gaur, D.; Batra, J.K.
    Role of aspartic acid 121 in human pancreatic ribonuclease catalysis (2005), Mol. Cell. Biochem., 275, 95-101.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.6.1.18 expression of wild-type and mutant enzymes in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
4.6.1.18 D121A Km-value for poly(C) is 2.4fold higher than wild-type value, Km-value for yeast tRNA is 1.25fold higher than wild-type value, turnover-number for poly(C) is 16.5fold lower than wild-type value, turnover-number for yeast tRNA is 1.3fold lower than wild-type value. ASp121 is crucial for the catalytic activity and may be involved in the depolymerization activity of the enzyme Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.6.1.18 1.7
-
poly(C) pH 7.5, 37°C, wild-type enzyme Homo sapiens
4.6.1.18 4
-
tRNA pH 7.5, 37°C, wild-type enzyme Homo sapiens
4.6.1.18 4
-
poly(C) pH 7.5, 37°C, mutant enzyme D121A Homo sapiens
4.6.1.18 5
-
tRNA pH 7.5, 37°C, mutant enzyme D121A Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.18 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.18 poly (C) + H2O
-
Homo sapiens 3'-CMP + 3'-phosphooligonucleotides
-
?
4.6.1.18 tRNA + H2O tRNA from yeast Homo sapiens 3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
?

Synonyms

EC Number Synonyms Comment Organism
4.6.1.18 HPR
-
Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.6.1.18 additional information
-
additional information
-
Homo sapiens