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Literature summary extracted from

  • Leung, Y.K.; Wong, K.F.; Lee, H.K.; Ho, J.W.
    Cloning and characterization of chironomidae ferrochelatase: copper activation of the purified ferrochelatase (2004), Mol. Cell. Biochem., 262, 225-231.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.98.1.1 Cu2+ 0.1 mM, causes 4fold increase in activity Chironomus sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.98.1.1 expression in Escherichia coli Chironomus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.98.1.1 Mg2+ 0.1 mM, reduces the enzymatic activity by more than 60% Chironomus sp.
4.98.1.1 Mn2+ 0.1 mM, reduces the enzymatic activity by more than 60% Chironomus sp.
4.98.1.1 Pb2+ 0.1 mM, reduces the enzymatic activity by more than 60% Chironomus sp.
4.98.1.1 Zn2+ 0.1 mM, inhibits over 90% of the enzyme activity Chironomus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.98.1.1 Cu2+ 0.1 mM, causes 4fold increase in activity Chironomus sp.
4.98.1.1 Fe2+ present in the cluster of the recombinant enzyme complex Chironomus sp.
4.98.1.1 Mg2+ 0.1 mM, reduces the enzymatic activity by more than 60% Chironomus sp.
4.98.1.1 Mn2+ 0.1 mM, reduces the enzymatic activity by more than 60% Chironomus sp.
4.98.1.1 Pb2+ 0.1 mM, reduces the enzymatic activity by more than 60% Chironomus sp.
4.98.1.1 Zn2+ 0.1 mM, inhibits over 90% of the enzyme activity Chironomus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.98.1.1 41200
-
 monomer, calculated from amino acid sequence Chironomus sp.
4.98.1.1 43000
-
 x * 43000, SDS-PAGE Chironomus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.98.1.1 protoporphyrin + Fe2+ Chironomus sp.
-
 protoheme + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.98.1.1 Chironomus sp. Q967G9 chironomid larvae
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.98.1.1
-
 Chironomus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.98.1.1 protoporphyrin + Fe2+
-
 Chironomus sp. protoheme + H+
-
 ?
4.98.1.1 protoporphyrin IX + Fe2+ pH 8.0, 37°C Chironomus sp. protoheme + 2 H+
-
 ?

Subunits

EC Number Subunits Comment Organism
4.98.1.1 ? x * 43000, SDS-PAGE Chironomus sp.

Synonyms

EC Number Synonyms Comment Organism
4.98.1.1 ferrochelatase
-
 Chironomus sp.