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Literature summary extracted from

  • Dhillon, N.K.; Sharma, S.; Khuller, G.K.
    Biochemical characterization of Ca2+/calmodulin dependent protein kinase from Candida albicans (2003), Mol. Cell. Biochem., 252, 183-191.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.17 Calmodulin dependent on Candida albicans

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.17 DTNB
-
 Candida albicans
2.7.11.17 KN-62 a Ca2+/calmodulin-dependent enzyme inhibitor Candida albicans
2.7.11.17 additional information no inhibition by H-7 and PKI Candida albicans
2.7.11.17 W-7 a Ca2+/calmodulin antagonist Candida albicans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.11.17 additional information
-
 additional information Ka for calmodulin is 8.3 nM Candida albicans
2.7.11.17 0.014
-
 ATP pH 7.0, 30°C Candida albicans
2.7.11.17 0.02
-
 syntide-2 pH 7.0, 30°C Candida albicans
2.7.11.17 0.063
-
 Lys-Lys-Ala-Leu-Arg-Arg-Gln-Glu-Ala-Val-Asp-Ala-Leu pH 7.0, 30°C Candida albicans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.11.17 cytosol
-
 Candida albicans 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.17 Ca2+ dependent on, activates Candida albicans
2.7.11.17 Mg2+ dependent on Candida albicans
2.7.11.17 Mn2+ partially stimulates Candida albicans
2.7.11.17 additional information little or no effect by Ni2+, Ba2+, Zn2+, Cu2+, and Cd2+ Candida albicans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.17 69000
-
 1 * 69000, SDS-PAGE Candida albicans
2.7.11.17 71000
-
 gel filtration Candida albicans

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.17 Candida albicans
-
 strain ATCC 3153, multifunctional enzyme
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.17 phosphoprotein the enzyme performs Ca2+/calmodulin-dependent autophosphorylation at a Ser residue Candida albicans

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.17 644fold from cytosol by ammonium sulfate fractionation, DEAE cellulose ion exchange chromatography, and gel filtration Candida albicans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.17 0.0035
-
 purified enzyme Candida albicans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.17 ATP + bovine serum albumin low activity Candida albicans ADP + phosphorylated bovine serum albumin
-
 ?
2.7.11.17 ATP + casein low activity Candida albicans ADP + phosphorylated casein
-
 ?
2.7.11.17 ATP + histone IIS
-
 Candida albicans ADP + phosphorylated histone IIS
-
 ?
2.7.11.17 ATP + Kemptide i.e. LRRASLG, low activity Candida albicans ADP + LRRAS(P)LG
-
 ?
2.7.11.17 ATP + KKALRRQETVDAL i.e. autocamtide-2 Candida albicans ADP + KKALRRQET(P)VDAL
-
 ?
2.7.11.17 ATP + phosvitin
-
 Candida albicans ADP + phosphorylated phosvitin
-
 ?
2.7.11.17 ATP + protamin low activity Candida albicans ADP + phosphorylated protamin
-
 ?
2.7.11.17 ATP + syntide-2 i.e. PLARTLSVAGLPGKK Candida albicans ADP + phosphorylated syntide-2
-
 ?
2.7.11.17 additional information the enzyme performs Ca2+/calmodulin-dependent autophosphorylation at a Ser residue Candida albicans ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.11.17 monomer 1 * 69000, SDS-PAGE Candida albicans

Synonyms

EC Number Synonyms Comment Organism
2.7.11.17 Ca2+/CaMPK
-
 Candida albicans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.17 30
-
 assay at Candida albicans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.11.17 55
-
 cytosolic extract, 60 min, inactivation Candida albicans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.17 8
-
-
 Candida albicans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.11.17 additional information
-
 broad range Candida albicans

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.17 ATP dependent on Candida albicans