EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.1 | expression in Escherichia coli strain BL21(DE3) as soluble intein fusion protein | Pyrococcus woesei |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.1 | Pyrococcus woesei | - |
DSM 3773 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.1 | recombinant soluble intein fusion protein partially from Escherichia coli strain BL21(DE3) by heat treatment at 75°C, cleavage of intein fusion protein | Pyrococcus woesei |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.1 | glycogen + H2O | 49.5% of the activity with starch | Pyrococcus woesei | maltose + ? | main product | ? | |
3.2.1.1 | additional information | no activity with pullulan | Pyrococcus woesei | ? | - |
? | |
3.2.1.1 | starch + H2O | preferred substrate | Pyrococcus woesei | maltose + ? | main product | ? |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.1 | additional information | - |
thermostable enzyme | Pyrococcus woesei |
3.2.1.1 | 90 | - |
partially purified recombinant enzyme, half-life is 11 h, at pH 5.6 in 0.05 M acetate buffer | Pyrococcus woesei |
3.2.1.1 | 110 | - |
partially purified recombinant enzyme, half-life is 3.5 h, at pH 5.6 in 0.05 M acetate buffer | Pyrococcus woesei |
3.2.1.1 | 120 | - |
partially purified recombinant enzyme, 2 h, at pH 5.6 in 0.05 M acetate buffer, 24% remaining activity | Pyrococcus woesei |