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Literature summary extracted from

  • Grzybowska, B.; Szweda, P.; Synowiecki, J.
    Cloning of the thermostable alpha-amylase gene from Pyrococcus woesei in Escherichia coli: isolation and some properties of the enzyme (2004), Mol. Biotechnol., 26, 101-110.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.1 expression in Escherichia coli strain BL21(DE3) as soluble intein fusion protein Pyrococcus woesei

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Pyrococcus woesei
-
DSM 3773
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.1 recombinant soluble intein fusion protein partially from Escherichia coli strain BL21(DE3) by heat treatment at 75°C, cleavage of intein fusion protein Pyrococcus woesei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 glycogen + H2O 49.5% of the activity with starch Pyrococcus woesei maltose + ? main product ?
3.2.1.1 additional information no activity with pullulan Pyrococcus woesei ?
-
?
3.2.1.1 starch + H2O preferred substrate Pyrococcus woesei maltose + ? main product ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.1 additional information
-
thermostable enzyme Pyrococcus woesei
3.2.1.1 90
-
partially purified recombinant enzyme, half-life is 11 h, at pH 5.6 in 0.05 M acetate buffer Pyrococcus woesei
3.2.1.1 110
-
partially purified recombinant enzyme, half-life is 3.5 h, at pH 5.6 in 0.05 M acetate buffer Pyrococcus woesei
3.2.1.1 120
-
partially purified recombinant enzyme, 2 h, at pH 5.6 in 0.05 M acetate buffer, 24% remaining activity Pyrococcus woesei