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Literature summary extracted from

  • Bruce, A.F.; Gounaris, K.
    Characterisation of a secreted N-acetyl-beta-hexosaminidase from Trichinella spiralis (2006), Mol. Biochem. Parasitol., 145, 84-93.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 N-acetyl-beta-D-galactosamine
-
 Trichinella spiralis
3.2.1.52 N-acetyl-beta-D-glucosamine
-
 Trichinella spiralis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.52 0.187
-
 N-acetyl-beta-D-glucosamine
-
 Trichinella spiralis
3.2.1.52 0.215
-
 N-acetyl-beta-D-galactosamine
-
 Trichinella spiralis
3.2.1.52 1.732
-
 N-acetyl-beta-D-glucosamine-6-sulfate
-
 Trichinella spiralis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 50000
-
 SDS-PAGE Trichinella spiralis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Trichinella spiralis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 affinity chromatography Trichinella spiralis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 additional information
-
 no specific activity on N-acetyl-alpha-D-glucosamine and N-acetyl-alpha-D-galactosamine at pH 4.4 and 37°C Trichinella spiralis
3.2.1.52 0.00002
-
 p-nitrophenyl-beta-D-mannose, pH 4.4, 37°C Trichinella spiralis
3.2.1.52 0.00007
-
 p-nitrophenyl-beta-D-glucose, pH 4.4, 37°C Trichinella spiralis
3.2.1.52 0.00024
-
 p-nitrophenyl-alpha-D-xylose, pH 4.4, 37°C Trichinella spiralis
3.2.1.52 0.00028
-
 p-nitrophenyl-beta-D-fucose, pH 4.4, 37°C Trichinella spiralis
3.2.1.52 0.00032
-
 p-nitrophenyl-beta-D-galactose, pH 4.4, 37°C Trichinella spiralis
3.2.1.52 0.00033
-
 p-nitrophenyl-beta-D-xylose, pH 4.4, 37°C Trichinella spiralis
3.2.1.52 0.00042
-
 N,N,N-triacetylchitotriose, pH 4.4, 37°C Trichinella spiralis
3.2.1.52 0.00043
-
 p-nitrophenyl-alpha-D-mannose, pH 4.4, 37°C Trichinella spiralis
3.2.1.52 0.00046
-
 p-nitrophenyl-alpha-D-fucose, pH 4.4, 37°C Trichinella spiralis
3.2.1.52 0.00066
-
 p-nitrophenyl-beta-D-glucuoronide, pH 4.4, 37°C Trichinella spiralis
3.2.1.52 0.00079
-
 N,N-diacetylchitobiose, pH 4.4, 37°C Trichinella spiralis
3.2.1.52 0.0018
-
 4-methylumbelliferyl-N-acetyl-beta-D-glucosamine-6-sulphate, pH 4.4, 37°C Trichinella spiralis
3.2.1.52 0.00185
-
 p-nitrophenyl-alpha-D-glucose, pH 4.4, 37°C Trichinella spiralis
3.2.1.52 0.00396
-
 4-methylumbelliferyl-N-acetyl-beta-D-galactosamine, pH 4.4, 37°C Trichinella spiralis
3.2.1.52 0.00832
-
 4-methylumbelliferyl-N-acetyl-beta-D-glucosamine, pH 4.4, 37°C Trichinella spiralis
3.2.1.52 0.01199
-
 p-nitrophenyl-N-acetyl-beta-D-galactosamine, pH 4.4, 37°C Trichinella spiralis
3.2.1.52 0.0126
-
 2-O(2-acet-aminido-2-deoxy-beta-D-glucopyranosyl)-D-mannose, pH 4.4, 37°C Trichinella spiralis
3.2.1.52 0.02908
-
 p-nitrophenyl-N-acetyl-beta-D-glucosamine, pH 4.4, 37°C Trichinella spiralis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 4-methylumbelliferyl-N-acetyl-beta-D-glucosamine-6-sulfate + H2O
-
 Trichinella spiralis 4-methylumbelliferone + N-acetyl-beta-D-glucosamine-6-sulfate
-
 ?
3.2.1.52 N,N',N''-triacetylchitotriose + H2O
-
 Trichinella spiralis ?
-
 ?
3.2.1.52 N,N'-diacetylchitobiose + H2O
-
 Trichinella spiralis ?
-
 ?
3.2.1.52 p-nitrophenyl-N-acetyl-beta-D-galactosamine + H2O
-
 Trichinella spiralis N-acetyl-beta-D-galactosamine + p-nitrophenol
-
 ?
3.2.1.52 p-nitrophenyl-N-acetyl-beta-D-glucosamine + H2O
-
 Trichinella spiralis N-acetyl-beta-D-glucosamine + p-nitrophenol
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.52 exo-beta-hexosaminidase
-
 Trichinella spiralis
3.2.1.52 N-acetyl-beta-hexosaminidase
-
 Trichinella spiralis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.52 54
-
 in citrate phosphate buffer with p-nitrophenyl-N-acetyl-beta-D-glucosamine as substrate Trichinella spiralis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.52 4 70 in citrate phosphate buffer with p-nitrophenyl-N-acetyl-beta-D-glucosamine as substrate Trichinella spiralis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 4.4
-
 in citrate phosphate buffer with p-nitrophenyl-N-acetyl-beta-D-glucosamine as substrate Trichinella spiralis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.52 2.4 7.4 in citrate phosphate buffer with p-nitrophenyl-N-acetyl-beta-D-glucosamine as substrate Trichinella spiralis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.52 1.17
-
 N-acetyl-beta-D-galactosamine
-
 Trichinella spiralis
3.2.1.52 15.75
-
 N-acetyl-beta-D-glucosamine
-
 Trichinella spiralis