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Literature summary extracted from

  • Dong, L.; Cheng, N.; Wang, M.W.; Zhang, J.; Shu, C.; Zhu, D.X.
    The leucyl aminopeptidase from Helicobacter pylori is an allosteric enzyme (2005), Microbiology, 151, 2017-2023.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.11.10 pharmacology the enzyme is a target for development of anti-Helicobacter pylori agents Helicobacter pylori

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.10 gene LAP, DNA and amino acid sequence determination and analysis, functional expression of His-tagged enzyme in Escherichia coli Helicobacter pylori

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.10 bestatin inhibits the enzyme activity and cell growth Helicobacter pylori
3.4.11.10 Ca2+ 78% inhibition at 0.1 mM, 94% inhibition at 1 mM, 96% inhibition at 10 mM Helicobacter pylori
3.4.11.10 EDTA 97% inhibition at 0.1 mM Helicobacter pylori
3.4.11.10 additional information no inhibition by PMSF Helicobacter pylori
3.4.11.10 Zn2+ 65% inhibition at 0.1 mM, 78% inhibition at 1 mM, 91% inhibition at 10 mM Helicobacter pylori

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.10 Co2+ highly activating, about 5fold at 1-10 mM Helicobacter pylori
3.4.11.10 Mg2+ activates 2.9fold at 1 mM, 3.7fold at 10 mM Helicobacter pylori
3.4.11.10 Mn2+ highly activating, 5.6fold at 1-10 mM Helicobacter pylori
3.4.11.10 Ni2+ activates 3.5fold at 1 mM, 3.8fold at 10 mM Helicobacter pylori

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.10 55000
-
 6 * 55000, recombinant LAP, SDS-PAGE Helicobacter pylori
3.4.11.10 340000
-
 recombinant enzyme, gel filtration Helicobacter pylori

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.10 Helicobacter pylori
-
 gene LAP
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.10 L-Leu-4-nitroanilide + H2O
-
 Helicobacter pylori L-Leu + 4-nitroaniline
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.10 hexamer 6 * 55000, recombinant LAP, SDS-PAGE Helicobacter pylori
3.4.11.10 More the monomeric enzyme is probably not active Helicobacter pylori

Synonyms

EC Number Synonyms Comment Organism
3.4.11.10 LAP
-
 Helicobacter pylori
3.4.11.10 Leucyl aminopeptidase
-
 Helicobacter pylori

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.10 45
-
 recombinant LAP Helicobacter pylori

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.11.10 15 100 temperature profile, about 60% of maximal activity at 15°C and 100°C, recombinant enzyme Helicobacter pylori

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.10 8
-
 recombinant LAP Helicobacter pylori

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.10 5.5 10 pH profile, about 40% of maximal activity at pH 5.5 and pH 10.0, rapid decline of activity at pH 5.0, recombinant enzyme Helicobacter pylori