BRENDA - Enzyme Database

Amino acid residues involved in cold adaptation of isocitrate lyase from a psychrophilic bacterium, Colwellia maris

Watanabe, S.; Takada, Y.; Microbiology 150, 3393-3403 (2004)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.1.3.1
expression in Escherichia coli
Colwellia maris
4.1.3.1
expression in Escherichia coli
Escherichia coli
Engineering
EC Number
Amino acid exchange
Commentary
Organism
4.1.3.1
Q207H
mutant with decreased activity between 10C and 25C
Colwellia maris
4.1.3.1
Q217K
mutant with decreased activity between 10C and 25C
Colwellia maris
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.25
-
isocitrate
10C
Escherichia coli
4.1.3.1
0.26
-
isocitrate
mutant Q217K, 10C
Colwellia maris
4.1.3.1
0.33
-
isocitrate
wild-type, 10C
Colwellia maris
4.1.3.1
0.47
-
isocitrate
wild-type, 20C
Colwellia maris
4.1.3.1
0.5
-
isocitrate
mutant Q217K, 30C
Colwellia maris
4.1.3.1
0.65
-
isocitrate
40C
Escherichia coli
4.1.3.1
0.76
-
isocitrate
mutant Q207H, 10C
Colwellia maris
4.1.3.1
2.07
-
isocitrate
mutant Q207H, 30C
Colwellia maris
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Colwellia maris
-
-
-
4.1.3.1
Escherichia coli
P0A9G6
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.1
-
Colwellia maris
4.1.3.1
-
Escherichia coli
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
4.1.3.1
10.5
-
mutant Q217K
Colwellia maris
4.1.3.1
14.5
-
mutant Q207H
Colwellia maris
4.1.3.1
14.6
-
wild-type
Colwellia maris
4.1.3.1
54
-
-
Escherichia coli
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
666268
Escherichia coli
glyoxylate + succinate
-
-
-
?
4.1.3.1
isocitrate
-
666268
Colwellia maris
glyoxylate + succinate
-
-
-
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
4.1.3.1
additional information
-
optimum temperature above 40C
Escherichia coli
4.1.3.1
20
25
wild type
Colwellia maris
4.1.3.1
27.5
30
mutant Q207H
Colwellia maris
4.1.3.1
30
32.5
mutant Q217K
Colwellia maris
Temperature Stability [C]
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
4.1.3.1
30
-
mutant Q207H, half time of thermal inactivation 58.4 s; mutant Q217K, half time of thermal inactivation 120.3 s; wild-type, half time of thermal inactivation 88.2 s
Colwellia maris
4.1.3.1
40
-
half time of thermal inactivation 161.8 s
Escherichia coli
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
6.8
-
wild-type
Colwellia maris
4.1.3.1
6.85
-
mutants Q207H and Q217k
Colwellia maris
4.1.3.1
6.9
-
-
Escherichia coli
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
expression in Escherichia coli
Colwellia maris
4.1.3.1
expression in Escherichia coli
Escherichia coli
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
4.1.3.1
Q207H
mutant with decreased activity between 10C and 25C
Colwellia maris
4.1.3.1
Q217K
mutant with decreased activity between 10C and 25C
Colwellia maris
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.25
-
isocitrate
10C
Escherichia coli
4.1.3.1
0.26
-
isocitrate
mutant Q217K, 10C
Colwellia maris
4.1.3.1
0.33
-
isocitrate
wild-type, 10C
Colwellia maris
4.1.3.1
0.47
-
isocitrate
wild-type, 20C
Colwellia maris
4.1.3.1
0.5
-
isocitrate
mutant Q217K, 30C
Colwellia maris
4.1.3.1
0.65
-
isocitrate
40C
Escherichia coli
4.1.3.1
0.76
-
isocitrate
mutant Q207H, 10C
Colwellia maris
4.1.3.1
2.07
-
isocitrate
mutant Q207H, 30C
Colwellia maris
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
-
Colwellia maris
4.1.3.1
-
Escherichia coli
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
4.1.3.1
10.5
-
mutant Q217K
Colwellia maris
4.1.3.1
14.5
-
mutant Q207H
Colwellia maris
4.1.3.1
14.6
-
wild-type
Colwellia maris
4.1.3.1
54
-
-
Escherichia coli
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
666268
Escherichia coli
glyoxylate + succinate
-
-
-
?
4.1.3.1
isocitrate
-
666268
Colwellia maris
glyoxylate + succinate
-
-
-
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
4.1.3.1
additional information
-
optimum temperature above 40C
Escherichia coli
4.1.3.1
20
25
wild type
Colwellia maris
4.1.3.1
27.5
30
mutant Q207H
Colwellia maris
4.1.3.1
30
32.5
mutant Q217K
Colwellia maris
Temperature Stability [C] (protein specific)
EC Number
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
4.1.3.1
30
-
mutant Q207H, half time of thermal inactivation 58.4 s; mutant Q217K, half time of thermal inactivation 120.3 s; wild-type, half time of thermal inactivation 88.2 s
Colwellia maris
4.1.3.1
40
-
half time of thermal inactivation 161.8 s
Escherichia coli
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
6.8
-
wild-type
Colwellia maris
4.1.3.1
6.85
-
mutants Q207H and Q217k
Colwellia maris
4.1.3.1
6.9
-
-
Escherichia coli