BRENDA - Enzyme Database

Characterization of an iron-regulated alpha-enolase of Bacteroides fragilis

Sijbrandi, R.; Den Blaauwen, T.; Tame, J.R.; Oudega, B.; Luirink, J.; Otto, B.R.; Microbes Infect. 7, 9-18 (2005)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.11
expression in Escherichia coli strain BL21 AI with an N-terminal 10His-tag
Bacteroides fragilis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.21
-
2-phosphoglyceric acid
37°C, in reaction buffer
Bacteroides fragilis
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.11
inner membrane
-
Bacteroides fragilis
-
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Fe2+
-
Bacteroides fragilis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
49000
-
SDS-PAGE
Bacteroides fragilis
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Bacteroides fragilis
-
phosphoenolpyruvate + H2O
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Bacteroides fragilis
Q8KNX9
strain BE1
-
4.2.1.11
Bacteroides fragilis YCH46
Q8KNX9
strain BE1
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
Ni2+-charged HiTrap chelating column
Bacteroides fragilis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
666254
Bacteroides fragilis
phosphoenolpyruvate + H2O
-
-
-
r
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
expression in Escherichia coli strain BL21 AI with an N-terminal 10His-tag
Bacteroides fragilis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.21
-
2-phosphoglyceric acid
37°C, in reaction buffer
Bacteroides fragilis
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
4.2.1.11
inner membrane
-
Bacteroides fragilis
-
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Fe2+
-
Bacteroides fragilis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.2.1.11
49000
-
SDS-PAGE
Bacteroides fragilis
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Bacteroides fragilis
-
phosphoenolpyruvate + H2O
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
Ni2+-charged HiTrap chelating column
Bacteroides fragilis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
666254
Bacteroides fragilis
phosphoenolpyruvate + H2O
-
-
-
r