Literature summary extracted from
Allen, A.G.; Lindsay, H.; Seilly, D.; Bolitho, S.; Peters, S.E.; Maskell, D.J.
Identification and characterisation of hyaluronate lyase from Streptococcus suis (2004), Microb. Pathog., 36, 327-335.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
4.2.2.1 |
gene hyl, DNA and amino acid sequence determination and analysis, overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) |
Streptococcus suis |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
4.2.2.1 |
additional information |
construction of an enzyme deficient strain by allelic relacement knockout of serotype 7 |
Streptococcus suis |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
4.2.2.1 |
54260 |
- |
x * 54260, sequence calculation |
Streptococcus suis |
4.2.2.1 |
126500 |
130000 |
amino acid sequence calculation and native PAGE |
Streptococcus suis |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
4.2.2.1 |
hyaluronic acid |
Streptococcus suis |
the enzyme is essential for growth on hyaluronate as carbon source |
hyaluronic acid oligomers |
- |
? |
|
4.2.2.1 |
additional information |
Streptococcus suis |
the enzyme is involved in development of meningitis in infected pigs |
? |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.2.2.1 |
Streptococcus suis |
Q8VLQ8 |
hyaluronidase precursor; gene hylAP1/7, serotype 7 |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
4.2.2.1 |
native enzyme from serotype 7 to over 95% purity, recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) |
Streptococcus suis |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
4.2.2.1 |
[hyaluronate]n = (4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine-[hyaluronate]n-m-1 + 2 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine + hyaluronate |
catalytic residues are Asn349, His399, and Tyr408 |
Streptococcus suis |
|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.2.2.1 |
hyaluronic acid |
the enzyme is essential for growth on hyaluronate as carbon source |
Streptococcus suis |
hyaluronic acid oligomers |
- |
? |
|
4.2.2.1 |
hyaluronic acid |
native and recombinant serotype 7 enzyme, human umbelical cord hyaluronate |
Streptococcus suis |
hyaluronic acid oligomers |
- |
? |
|
4.2.2.1 |
additional information |
the enzyme is involved in development of meningitis in infected pigs |
Streptococcus suis |
? |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
4.2.2.1 |
? |
x * 54260, sequence calculation |
Streptococcus suis |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
4.2.2.1 |
hyaluronidase |
- |
Streptococcus suis |
4.2.2.1 |
HylA7 |
- |
Streptococcus suis |
Temperature Optimum [°C]
EC Number |
Temperature Optimum [°C] |
Temperature Optimum Maximum [°C] |
Comment |
Organism |
---|
4.2.2.1 |
37 |
- |
assay at |
Streptococcus suis |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
4.2.2.1 |
5.5 |
- |
assay at |
Streptococcus suis |