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Literature summary extracted from

  • Ueda, N.; Okamoto, Y.; Morishita, J.
    N-acylphosphatidylethanolamine-hydrolyzing phospholipase D: a novel enzyme of the beta-lactamase fold family releasing anandamide and other N-acylethanolamines (2005), Life Sci., 77, 1750-1758.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.54 spermine
-
 Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.4 overexpression in COS-7 cells Rattus norvegicus
3.1.4.54 expression in COS-7 cells Mus musculus
3.1.4.54 overexpression in COS-7 cells Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.54 0.008
-
 N-palmitoylphosphatidylethanolamine 37°C, pH not specified in the publication, Ca2+ stimulated enzyme Rattus norvegicus
3.1.4.54 0.045
-
 N-palmitoylphosphatidylethanolamine 37°C, pH not specified in the publication, Triton X-100 stimulated enzyme Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.54 Ba2+ stimulated up to 30fold by millimolar order of Ca2+. This effect is fully substituted by other divalent cations such as Co2+, Mg2+, Mn2+, Ba2+, Sr2+ and Ni2+ Rattus norvegicus
3.1.4.54 Ca2+ stimulated up to 30fold by millimolar order of Ca2+. This effect is fully substituted by other divalent cations such as Co2+, Mg2+, Mn2+, Ba2+, Sr2+ and Ni2+ Rattus norvegicus
3.1.4.54 Co2+ stimulated up to 30fold by millimolar order of Ca2+. This effect is fully substituted by other divalent cations such as Co2+, Mg2+, Mn2+, Ba2+, Sr2+ and Ni2+ Rattus norvegicus
3.1.4.54 Mg2+ stimulated up to 30fold by millimolar order of Ca2+. This effect is fully substituted by other divalent cations such as Co2+, Mg2+, Mn2+, Ba2+, Sr2+ and Ni2+ Rattus norvegicus
3.1.4.54 Mn2+ stimulated up to 30fold by millimolar order of Ca2+. This effect is fully substituted by other divalent cations such as Co2+, Mg2+, Mn2+, Ba2+, Sr2+ and Ni2+ Rattus norvegicus
3.1.4.54 Ni2+ stimulated up to 30fold by millimolar order of Ca2+. This effect is fully substituted by other divalent cations such as Co2+, Mg2+, Mn2+, Ba2+, Sr2+ and Ni2+ Rattus norvegicus
3.1.4.54 Sr2+ stimulated up to 30fold by millimolar order of Ca2+. This effect is fully substituted by other divalent cations such as Co2+, Mg2+, Mn2+, Ba2+, Sr2+ and Ni2+ Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.54 46000
-
 x * 46000, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.54 N-arachidonoylphosphatidylethanolamine + H2O Mus musculus the enzyme is involved in the biosynthesis of anandamide, an endocannabinoid that belongs to the class of bioactive, long-chain N-acylethanolamines N-arachidonoylethanolamine + phosphatidic acid N-arachidonoylethanolamine i.e. anandamide ?
3.1.4.54 N-arachidonoylphosphatidylethanolamine + H2O Rattus norvegicus the enzyme is involved in the biosynthesis of anandamide, an endocannabinoid that belongs to the class of bioactive, long-chain N-acylethanolamines N-arachidonoylethanolamine + phosphatidic acid N-arachidonoylethanolamine i.e. anandamide ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.4 Rattus norvegicus
-
-
-
3.1.4.54 Mus musculus Q8BH82
-
-
3.1.4.54 Rattus norvegicus Q769K2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.4
-
 Rattus norvegicus
3.1.4.54
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.4 brain
-
 Rattus norvegicus
-
3.1.4.4 heart
-
 Rattus norvegicus
-
3.1.4.4 kidney
-
 Rattus norvegicus
-
3.1.4.4 testis
-
 Rattus norvegicus
-
3.1.4.54 brain widely distributed with higher specific activities in brain, kidney and testis Mus musculus
-
3.1.4.54 heart
-
 Rattus norvegicus
-
3.1.4.54 kidney widely distributed with higher specific activities in brain, kidney and testis Mus musculus
-
3.1.4.54 testis widely distributed with higher specific activities in brain, kidney and testis Mus musculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.54 0.00002
-
 the endogenous NAPE-PLD activity with the homogenates of COS-7 cells transfected with the insert-free vector, pH and temperature not specified in the publication Mus musculus
3.1.4.54 0.019
-
 hydrolysis of N-palmitoylphosphatidylethanolamine, homogenates of transfected cells, pH and temperature not specified in the publication Mus musculus
3.1.4.54 0.406
-
 37°C, pH and temperature not specified in the publication Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.4 N-acylphosphatidylethanolamine + H2O
-
 Rattus norvegicus N-acylethanolamine + phosphatidate
-
 ?
3.1.4.54 additional information the enzyme hydrolyzes N-acyl-2-lysophosphatidylamines at a much lower rate than N-acyl-phosphatidylethanolamines Mus musculus ?
-
 ?
3.1.4.54 N-arachidonoylphosphatidylethanolamine + H2O the enzyme is involved in the biosynthesis of anandamide, an endocannabinoid that belongs to the class of bioactive, long-chain N-acylethanolamines Mus musculus N-arachidonoylethanolamine + phosphatidic acid N-arachidonoylethanolamine i.e. anandamide ?
3.1.4.54 N-arachidonoylphosphatidylethanolamine + H2O the enzyme is involved in the biosynthesis of anandamide, an endocannabinoid that belongs to the class of bioactive, long-chain N-acylethanolamines Rattus norvegicus N-arachidonoylethanolamine + phosphatidic acid N-arachidonoylethanolamine i.e. anandamide ?
3.1.4.54 N-arachidonoylphosphatidylethanolamine + H2O Km values and Vmax values for N-palmitoylphosphatidylethanolamine, N-arachidonoylphosphatidylethanolamine, N-oleoylphosphatidylethanolamine, and N-stearoylphosphatidylethanolamine are similar. The enzyme does not have selectivity for N-acyl groups of N-acylphosphatidylamines Mus musculus N-arachidonoylethanolamine + phosphatidate
-
 ?
3.1.4.54 N-oleoylphosphatidylethanolamine + H2O Km values and Vmax values for N-palmitoylphosphatidylethanolamine, N-arachidonoylphosphatidylethanolamine, N-oleoylphosphatidylethanolamine, and N-stearoylphosphatidylethanolamine are similar. The enzyme does not have selectivity for N-acyl groups of N-acylphosphatidylamines Mus musculus N-oleoylethanolamine + phosphatidate
-
 ?
3.1.4.54 N-palmitoylphosphatidylethanolamine + H2O
-
 Rattus norvegicus N-palmitoylethanolamine + phosphatidate
-
 ?
3.1.4.54 N-palmitoylphosphatidylethanolamine + H2O Km values and Vmax values for N-palmitoyl-phosphatidylethanolamine, N-arachidonoyl-phosphatidylethanolamine, N-oleoyl-phosphatidylethanolamine, and N-stearoyl-phosphatidylethanolamine are similar. The enzyme does not have selectivity for N-acyl groups of N-acylphosphatidylamines Mus musculus N-palmitoylethanolamine + phosphatidate
-
 ?
3.1.4.54 N-stearoylphosphatidylethanolamine + H2O Km values and Vmax values for N-palmitoylphosphatidylethanolamine, N-arachidonoylphosphatidylethanolamine, N-oleoylphosphatidylethanolamine, and N-stearoylphosphatidylethanolamine are similar. The enzyme does not have selectivity for N-acyl groups of N-acylphosphatidylamines Mus musculus N-stearoylethanolamine + phosphatidate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.54 ? x * 46000, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
3.1.4.4 N-acylphosphatidylethanolamine-hydrolyzing phospholipase D
-
 Rattus norvegicus
3.1.4.54 NAPE-PLD
-
 Mus musculus
3.1.4.54 NAPE-PLD
-
 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.54 7.5
-
-
 Rattus norvegicus