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Literature summary extracted from

  • Vigdorovich, V.; Strong, R.K.; Miller, A.D.
    Expression and characterization of a soluble, active form of the jaagsiekte sheep retrovirus receptor, Hyal2 (2005), J. Virol., 79, 79-86.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.35 expression of His6-tagged viral enzyme via baculovirus transfection in Spodoptera frugiperda Sf9 insect cells, the His-tag replaces the GPI anchor of the enzyme to make it soluble jaagsiekte sheep retrovirus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.35 jaagsiekte sheep retrovirus
-
 i.e. JSRV
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.35 recombinant His6-tagged viral enzyme from insect cells by metal affinity chromatography, ultrafiltration, and gel filtration jaagsiekte sheep retrovirus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.35 additional information during virus maturation in the host cell, the enzyme is located at the cell surface by a GPI-anchor jaagsiekte sheep retrovirus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.35 hyaluronan + H2O the enzyme shows low activity, degradation jaagsiekte sheep retrovirus hyaluronan oligomers
-
 ?
3.2.1.35 additional information the jaagsiekte sheep retrovirus receptor Hyal2 interacts specifically with the virus particles of the amphotropic murine leukemia virus-pseudotype vectors/JSRV-pseudotype vector envelope protein, binding kinetics, the binding inhibits virus entry into cells, overview jaagsiekte sheep retrovirus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.35 Hyal2
-
 jaagsiekte sheep retrovirus
3.2.1.35 hyaluronidase 2
-
 jaagsiekte sheep retrovirus
3.2.1.35 jaagsiekte sheep retrovirus receptor
-
 jaagsiekte sheep retrovirus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.35 37
-
 assay at jaagsiekte sheep retrovirus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.35 5.5
-
-
 jaagsiekte sheep retrovirus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.35 3.8 11 the enzyme is active over a wide pH range jaagsiekte sheep retrovirus