Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Qian, J.H.; Xu, J.H.
    Catalytic performance of a highly enantioselective (R)-ester hydrolase from a new isolate Acinetobacter sp. CGMCC 0789 (2004), J. Mol. Catal. B, 27, 227-232.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.6 benzethonium chloride i.e. (diisobutyl phenoxyethoxyethyl) dimethyl benzylammonium chloride, up to 2.5fold enhancement of activity Acinetobacter sp.
3.1.1.6 bis(2-ethylhexyl) sodium sulfosuccinate 138% of initial activity Acinetobacter sp.
3.1.1.6 bis(octadecyl) ammonium chloride 246% of initial activity Acinetobacter sp.
3.1.1.6 cetyltrimethyl ammonium bromide 184% of initial activity Acinetobacter sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.6 Cu2+
-
 Acinetobacter sp.
3.1.1.6 Fe3+
-
 Acinetobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.6 32.5
-
 p-nitrophenyl acetate pH 7.0, 25°C, presence of benzethonium chloride Acinetobacter sp.
3.1.1.6 385
-
 p-nitrophenyl acetate pH 7.0, 25°C, absence of benzethonium chloride Acinetobacter sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.6 Ag+ stimulation Acinetobacter sp.
3.1.1.6 Ba2+ stimulation Acinetobacter sp.
3.1.1.6 Ca2+ up to 150% stimulation Acinetobacter sp.
3.1.1.6 Co2+ stimulation Acinetobacter sp.
3.1.1.6 Mg2+ stimulation Acinetobacter sp.
3.1.1.6 Mn2+ stimulation Acinetobacter sp.
3.1.1.6 Pb2+ stimulation Acinetobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.6 20000
-
 x * 20000, SDS-PAGE Acinetobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.6 Acinetobacter sp.
-
-
-
3.1.1.6 Acinetobacter sp. CGMCC 0789
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.6 partial Acinetobacter sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.6 0.082
-
 substrate p-nitrophenyl laurate, pH 7.0, 25°C Acinetobacter sp.
3.1.1.6 0.251
-
 substrate p-nitrophenyl acetate, pH 7.0, 25°C Acinetobacter sp.
3.1.1.6 0.297
-
 substrate p-nitrophenyl decanoate, pH 7.0, 25°C Acinetobacter sp.
3.1.1.6 0.352
-
 substrate p-nitrophenyl hexanoate, pH 7.0, 25°C Acinetobacter sp.
3.1.1.6 0.369
-
 substrate p-nitrophenyl propionate, pH 7.0, 25°C Acinetobacter sp.
3.1.1.6 0.474
-
 substrate p-nitrophenyl butyrate, pH 7.0, 25°C Acinetobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.6 (4R)-hydroxy-3-methyl-2-(2-propynyl)-cyclopent-2-enone acetate + H2O highly enantioselective enzyme, enantioselectivity raises by 1.2fold from 16 to 20 when reaction temperature is raised from 30°C to 60°C. Both enantioselectivity and activity are raised by addition of the cationic detergent benzethonium chloride Acinetobacter sp. ?
-
 ?
3.1.1.6 (4R)-hydroxy-3-methyl-2-(2-propynyl)-cyclopent-2-enone acetate + H2O highly enantioselective enzyme, enantioselectivity raises by 1.2fold from 16 to 20 when reaction temperature is raised from 30°C to 60°C. Both enantioselectivity and activity are raised by addition of the cationic detergent benzethonium chloride Acinetobacter sp. CGMCC 0789 ?
-
 ?
3.1.1.6 p-nitrophenyl acetate + H2O
-
 Acinetobacter sp. p-nitrophenol + acetate
-
 ?
3.1.1.6 p-nitrophenyl acetate + H2O
-
 Acinetobacter sp. CGMCC 0789 p-nitrophenol + acetate
-
 ?
3.1.1.6 p-nitrophenyl butyrate + H2O
-
 Acinetobacter sp. p-nitrophenol + butyrate
-
 ?
3.1.1.6 p-nitrophenyl decanoate + H2O
-
 Acinetobacter sp. p-nitrophenol + decanoate
-
 ?
3.1.1.6 p-nitrophenyl hexanoate + H2O
-
 Acinetobacter sp. p-nitrophenol + hexanoate
-
 ?
3.1.1.6 p-nitrophenyl hexanoate + H2O
-
 Acinetobacter sp. CGMCC 0789 p-nitrophenol + hexanoate
-
 ?
3.1.1.6 p-nitrophenyl laurate + H2O
-
 Acinetobacter sp. p-nitrophenol + laurate
-
 ?
3.1.1.6 p-nitrophenyl laurate + H2O
-
 Acinetobacter sp. CGMCC 0789 p-nitrophenol + laurate
-
 ?
3.1.1.6 p-nitrophenyl propionate + H2O
-
 Acinetobacter sp. p-nitrophenol + propionate
-
 ?
3.1.1.6 p-nitrophenyl propionate + H2O
-
 Acinetobacter sp. CGMCC 0789 p-nitrophenol + propionate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.6 ? x * 20000, SDS-PAGE Acinetobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.6 60
-
-
 Acinetobacter sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.6 60
-
 1 h, 60% residual activity Acinetobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.6 8
-
-
 Acinetobacter sp.