EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.20 | gene malA, DNA and amino acid sequence analysis, phylogenetic tree, overexpression of wild-type and selenomethionine-labeled enzyme in Escherichia coli strain NF1830 | Saccharolobus solfataricus |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.2.1.20 | purified recombinant wild-type and selenomethionine-labeled enzyme, hanging drop vapour diffusion method, hexameric crystal form, X-ray diffraction structure determination and analysis of the different crystal forms at 2.5 A resolution, structure modelling of domains, full-length enzyme, and active site | Saccharolobus solfataricus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.20 | additional information | - |
additional information | kinetics | Saccharolobus solfataricus | |
3.2.1.20 | 2 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 4.5, 85°C, recombinant enzyme | Saccharolobus solfataricus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 470000 | - |
recombinant enzyme, native PAGE | Saccharolobus solfataricus |
3.2.1.20 | 480000 | - |
recombinant enzyme, gel filtration | Saccharolobus solfataricus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.20 | maltose + H2O | Saccharolobus solfataricus | - |
alpha-D-glucose + D-glucose | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.20 | Saccharolobus solfataricus | P0CD66 | gene malA | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.20 | recombinant wild-type and selenomethionine-labeled enzyme from Escherichia coli strain NF1830 by ammonium sulfate fractionation, ion exchange chromatography, and gel filtration | Saccharolobus solfataricus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.2.1.20 | maltotetraose + 3 H2O = 4 alpha-D-glucose | substrate recognition and catalytic mechanism, active site structure, residues R400, D87, W284, M321, F327 are involved in formation of the +1 subsite in the GH31 alpha-glucosidase substrate binding | Saccharolobus solfataricus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.20 | 4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Saccharolobus solfataricus | 4-nitrophenol + alpha-D-glucose | - |
? | |
3.2.1.20 | maltose + H2O | - |
Saccharolobus solfataricus | alpha-D-glucose + D-glucose | - |
? | |
3.2.1.20 | maltose + H2O | preferred substrate, Asp251 and Trp284 interact with the position 6 OH group of the substrate | Saccharolobus solfataricus | alpha-D-glucose + D-glucose | - |
? | |
3.2.1.20 | additional information | substrate specificity, overview | Saccharolobus solfataricus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.20 | hexamer | domain structure analysis, quarternary organization, comparison to alpha-xylosidase Yic1 | Saccharolobus solfataricus |
3.2.1.20 | More | monomer fold, overview | Saccharolobus solfataricus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.20 | AG | - |
Saccharolobus solfataricus |
3.2.1.20 | MalA | - |
Saccharolobus solfataricus |
3.2.1.20 | More | the enzyme belongs to the glycoside hydrolase family 31, GH31 | Saccharolobus solfataricus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 85 | - |
assay at | Saccharolobus solfataricus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.20 | 33 | - |
4-nitrophenyl alpha-D-glucopyranoside | pH 4.5, 85°C, recombinant enzyme | Saccharolobus solfataricus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 4.5 | - |
assay at | Saccharolobus solfataricus |