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Literature summary extracted from

  • Sims, P.A.; Menefee, A.L.; Larsen, T.M.; Mansoorabadi, S.O.; Reed, G.H.
    Structure and catalytic properties of an engineered heterodimer of enolase composed of one active and one inactive subunit (2006), J. Mol. Biol., 355, 422-431.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.11 expression in Escherichia coli Saccharomyces cerevisiae

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.11 engineered K345A/N80D/N126D heterodimer in complex with substrate/product, 1.85A resolution, batch method Saccharomyces cerevisiae

Protein Variants

EC Number Protein Variants Comment Organism
4.2.1.11 E211Q inactive, but properly folded Saccharomyces cerevisiae
4.2.1.11 K345A inactive Saccharomyces cerevisiae
4.2.1.11 K345A/N80D/N126D heterodimer with one inactive K345A subunit and one active N80D and N126D subunit Saccharomyces cerevisiae
4.2.1.11 N80D/N126D mutant with surface mutations to facilitate ion-exchange chromatographic separation Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.11 0.043
-
 2-phospho-D-glycerate wild type, 25°C, Mg2+, pH 7.5 Saccharomyces cerevisiae
4.2.1.11 0.05
-
 2-phospho-D-glycerate K345A/N80D/N126D, 25°C, Mg2+, pH 7.5 Saccharomyces cerevisiae
4.2.1.11 0.057
-
 2-phospho-D-glycerate N80D/N126D, 25°C, Mg2+, pH 7.5 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.11 2-phospho-D-glycerate Saccharomyces cerevisiae
-
 phosphoenolpyruvate + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.11 Saccharomyces cerevisiae P00924
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.11 ammonium sulfate precipitation and gel filtration Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.11 2-phospho-D-glycerate
-
 Saccharomyces cerevisiae phosphoenolpyruvate + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
4.2.1.11 heterodimer engineered type Saccharomyces cerevisiae
4.2.1.11 homodimer N80D/N126D Saccharomyces cerevisiae