BRENDA - Enzyme Database

Structure and catalytic properties of an engineered heterodimer of enolase composed of one active and one inactive subunit

Sims, P.A.; Menefee, A.L.; Larsen, T.M.; Mansoorabadi, S.O.; Reed, G.H.; J. Mol. Biol. 355, 422-431 (2006)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.11
expression in Escherichia coli
Saccharomyces cerevisiae
Crystallization (Commentary)
EC Number
Crystallization
Organism
4.2.1.11
engineered K345A/N80D/N126D heterodimer in complex with substrate/product, 1.85A resolution, batch method
Saccharomyces cerevisiae
Engineering
EC Number
Amino acid exchange
Commentary
Organism
4.2.1.11
E211Q
inactive, but properly folded
Saccharomyces cerevisiae
4.2.1.11
K345A
inactive
Saccharomyces cerevisiae
4.2.1.11
K345A/N80D/N126D
heterodimer with one inactive K345A subunit and one active N80D and N126D subunit
Saccharomyces cerevisiae
4.2.1.11
N80D/N126D
mutant with surface mutations to facilitate ion-exchange chromatographic separation
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.043
-
2-phospho-D-glycerate
wild type, 25C, Mg2+, pH 7.5
Saccharomyces cerevisiae
4.2.1.11
0.05
-
2-phospho-D-glycerate
K345A/N80D/N126D, 25C, Mg2+, pH 7.5
Saccharomyces cerevisiae
4.2.1.11
0.057
-
2-phospho-D-glycerate
N80D/N126D, 25C, Mg2+, pH 7.5
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Saccharomyces cerevisiae
-
phosphoenolpyruvate + H2O
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Saccharomyces cerevisiae
P00924
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
ammonium sulfate precipitation and gel filtration
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
666065
Saccharomyces cerevisiae
phosphoenolpyruvate + H2O
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
heterodimer
engineered type
Saccharomyces cerevisiae
4.2.1.11
homodimer
N80D/N126D
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
expression in Escherichia coli
Saccharomyces cerevisiae
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
4.2.1.11
engineered K345A/N80D/N126D heterodimer in complex with substrate/product, 1.85A resolution, batch method
Saccharomyces cerevisiae
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
4.2.1.11
E211Q
inactive, but properly folded
Saccharomyces cerevisiae
4.2.1.11
K345A
inactive
Saccharomyces cerevisiae
4.2.1.11
K345A/N80D/N126D
heterodimer with one inactive K345A subunit and one active N80D and N126D subunit
Saccharomyces cerevisiae
4.2.1.11
N80D/N126D
mutant with surface mutations to facilitate ion-exchange chromatographic separation
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.2.1.11
0.043
-
2-phospho-D-glycerate
wild type, 25C, Mg2+, pH 7.5
Saccharomyces cerevisiae
4.2.1.11
0.05
-
2-phospho-D-glycerate
K345A/N80D/N126D, 25C, Mg2+, pH 7.5
Saccharomyces cerevisiae
4.2.1.11
0.057
-
2-phospho-D-glycerate
N80D/N126D, 25C, Mg2+, pH 7.5
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Saccharomyces cerevisiae
-
phosphoenolpyruvate + H2O
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
ammonium sulfate precipitation and gel filtration
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
666065
Saccharomyces cerevisiae
phosphoenolpyruvate + H2O
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
heterodimer
engineered type
Saccharomyces cerevisiae
4.2.1.11
homodimer
N80D/N126D
Saccharomyces cerevisiae