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Literature summary extracted from

  • Bagautdinov, B.; Kuroishi, C.; Sugahara, M.; Kunishima, N.
    Crystal structures of biotin protein ligase from Pyrococcus horikoshii OT3 and its complexes: structural basis of biotin activation (2005), J. Mol. Biol., 353, 322-333.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.15 overexpression of the enzyme comprising residues 1-125 in Escherichia coli strain BL21 Pyrococcus horikoshii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.3.4.15 purified recombinant native and selenomethionine-labeled enzyme comprising residues 1-125, sitting drop method, 9.87 mg/ml protein in 20 mM Tris-HCl, pH 8.0, 500 mM NaCl, 0.001 ml protein solution is mixed with 0.001 ml precipitation solution containing 10.5% PEG 2000, 0.1 M acetate-NaOH, pH 5.5, the drop is overlaid with a 1:1 mixture of silicone and paraffin oil allowing slow water evaporation from the drop, 22°C, preliminary X-ray diffraction structure determination and analysis at 1.45-1.6 A resolution Pyrococcus horikoshii

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.15 Pyrococcus horikoshii
-
 strain OT3
-
6.3.4.15 Pyrococcus horikoshii OT-3
-
 strain OT3
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.15 recombinant enzyme comprising residues 1-125 from Escherichia coli strain BL21 Pyrococcus horikoshii

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.4.15 ATP + biotin + [biotin carboxyl-carrier protein]-L-lysine = AMP + diphosphate + [biotin carboxyl-carrier protein]-N6-biotinyl-L-lysine reaction mechanism involving Lys111, formation of a biotinyl-5'-AMP intermediate, active site structure, ligand binding to the active site loop, overview Pyrococcus horikoshii
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] biotinylation of the biotin carboxyl carrier protein subunit of acetyl-CoA carboxylase Pyrococcus horikoshii AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?
6.3.4.15 ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] biotinylation of the biotin carboxyl carrier protein subunit of acetyl-CoA carboxylase Pyrococcus horikoshii OT-3 AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.15 dimer the functional unit is a dimer, a subunit contains a catalytic N-terminal domain and a C-terminal domain, structure analysis Pyrococcus horikoshii

Synonyms

EC Number Synonyms Comment Organism
6.3.4.15 biotin-protein ligase
-
 Pyrococcus horikoshii

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.4.15 ATP
-
 Pyrococcus horikoshii