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Literature summary extracted from

  • Varrot, A.; Yip, V.L.; Li, Y.; Rajan, S.S.; Yang, X.; Anderson, W.F.; Thompson, J.; Withers, S.G.; Davies, G.J.
    NAD+ and Metal-ion Dependent Hydrolysis by Family 4 Glycosidases: Structural Insight into Specificity for Phospho-b-D-glucosides (2005), J. Mol. Biol., 346, 423-435.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.86 hanging-drop vapor-diffusion method, crystal structure of the native selenomethionine-substituted enzyme (2.85 A resolution) and of the enzyme in complex with NAD+, Mn2+ and glucose 6-phosphate (2.55 A resolution) Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.86 0.041
-
 p-nitrophenyl-beta-D-glucopyranoside 6-phosphate pH 7.5, 50°C Thermotoga maritima
3.2.1.86 0.206
-
 p-nitrophenyl 6-phospho-beta-D-thioglucoside pH 7.5, 50°C Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.86 Thermotoga maritima Q9X108 recombinant
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.86 p-nitrophenyl 6-phospho-beta-D-thioglucoside + H2O
-
 Thermotoga maritima p-nitrophenol + 6-phospho-beta-D-thioglucose
-
 ?
3.2.1.86 p-nitrophenyl-beta-D-glucopyranoside 6-phosphate + H2O
-
 Thermotoga maritima p-nitrophenol + beta-D-glucopyranose 6-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.86 BglT
-
 Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.86 1.6
-
 p-nitrophenyl 6-phospho-beta-D-thioglucoside pH 7.5, 50°C Thermotoga maritima
3.2.1.86 1.9
-
 p-nitrophenyl-beta-D-glucopyranoside 6-phosphate pH 7.5, 50°C Thermotoga maritima