BRENDA - Enzyme Database

Expression, purification and the 1.8 angstroms resolution crystal structure of human neuron specific enolase

Chai, G.; Brewer, J.M.; Lovelace, L.L.; Aoki, T.; Minor, W.; Lebioda, L.; J. Mol. Biol. 341, 1015-1021 (2004)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.2.1.11
expression in Escherichia coli strain JM109 with C-terminal His-tag
Homo sapiens
Crystallization (Commentary)
EC Number
Crystallization
Organism
4.2.1.11
asymmetric complex NSE*Mg2SO4/NSE*MgCl, pH 7.0, large orthorhombic plates, structure by molecular replacement
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
Mg2+
at high concentrations
Homo sapiens
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
-
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Homo sapiens
-
phosphoenolpyruvate + H2O
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.2.1.11
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.2.1.11
Ni-NTA Sepharose, ion-exchange chromatography, ammonium sulfate precipitation
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
brain
-
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
666023
Homo sapiens
phosphoenolpyruvate + H2O
-
-
-
r
Subunits
EC Number
Subunits
Commentary
Organism
4.2.1.11
dimer
-
Homo sapiens
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
4.2.1.11
Homo sapiens
-
-
4.8
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
expression in Escherichia coli strain JM109 with C-terminal His-tag
Homo sapiens
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
4.2.1.11
asymmetric complex NSE*Mg2SO4/NSE*MgCl, pH 7.0, large orthorhombic plates, structure by molecular replacement
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.2.1.11
Mg2+
at high concentrations
Homo sapiens
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.2.1.11
Mg2+
-
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
4.2.1.11
2-phospho-D-glycerate
Homo sapiens
-
phosphoenolpyruvate + H2O
-
-
r
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.2.1.11
Ni-NTA Sepharose, ion-exchange chromatography, ammonium sulfate precipitation
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.2.1.11
brain
-
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.2.1.11
2-phospho-D-glycerate
-
666023
Homo sapiens
phosphoenolpyruvate + H2O
-
-
-
r
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.2.1.11
dimer
-
Homo sapiens
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
4.2.1.11
Homo sapiens
-
-
4.8