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Literature summary extracted from

  • Chai, G.; Brewer, J.M.; Lovelace, L.L.; Aoki, T.; Minor, W.; Lebioda, L.
    Expression, purification and the 1.8 angstroms resolution crystal structure of human neuron specific enolase (2004), J. Mol. Biol., 341, 1015-1021.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.1.11 expression in Escherichia coli strain JM109 with C-terminal His-tag Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.2.1.11 asymmetric complex NSE*Mg2SO4/NSE*MgCl, pH 7.0, large orthorhombic plates, structure by molecular replacement Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.11 Mg2+ at high concentrations Homo sapiens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.11 Mg2+
-
 Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.11 2-phospho-D-glycerate Homo sapiens
-
 phosphoenolpyruvate + H2O
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.11 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.11 Ni-NTA Sepharose, ion-exchange chromatography, ammonium sulfate precipitation Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.1.11 brain
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.11 2-phospho-D-glycerate
-
 Homo sapiens phosphoenolpyruvate + H2O
-
 r

Subunits

EC Number Subunits Comment Organism
4.2.1.11 dimer
-
 Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
4.2.1.11 human neuron-specific enolase
-
 Homo sapiens
4.2.1.11 NSE
-
 Homo sapiens

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.1.11 Homo sapiens
-
-
 4.8