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Literature summary extracted from

  • Kim, D.Y.; Kim, H.C.; Kim, S.Y.; Rhee, Y.H.
    Molecular characterization of extracellular medium-chain-length poly(3-hydroxyalkanoate) depolymerase genes from Pseudomonas alcaligenes strains (2005), J. Microbiol., 43, 285-294.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.76 gene phaZ, DNA and amino acid sequence determination and analysis, expression and subcloning in Escherichia coli strain DH5alpha Pseudomonas alcaligenes

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.76 4-hydroxymercuribenzoate
-
 Pseudomonas alcaligenes
3.1.1.76 Acetic anhydride
-
 Pseudomonas alcaligenes
3.1.1.76 diisopropylfluorophosphate
-
 Pseudomonas alcaligenes
3.1.1.76 EDTA
-
 Pseudomonas alcaligenes
3.1.1.76 iodoacetamide
-
 Pseudomonas alcaligenes
3.1.1.76 additional information no inhibition by DTT; no inhibition by DTT, sodium azide, and iododacetamide Pseudomonas alcaligenes
3.1.1.76 N-bromosuccimide
-
 Pseudomonas alcaligenes
3.1.1.76 N-bromosuccinimide
-
 Pseudomonas alcaligenes
3.1.1.76 PMSF
-
 Pseudomonas alcaligenes
3.1.1.76 Sodium azide
-
 Pseudomonas alcaligenes
3.1.1.76 Triton X-100
-
 Pseudomonas alcaligenes
3.1.1.76 Tween 80
-
 Pseudomonas alcaligenes

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.76 extracellular
-
 Pseudomonas alcaligenes
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.76 28000
-
 x * 28000, SDS-PAGE Pseudomonas alcaligenes
3.1.1.76 30323
-
 x * 30323, amino acid sequence calculation Pseudomonas alcaligenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.76 poly(3-hydroxyalkanoate) + H2O Pseudomonas alcaligenes
-
 oligo(3-hydroxyalkanoate) + 3-hydroxyalkanoate
-
 ?
3.1.1.76 poly(3-hydroxyalkanoate) + H2O Pseudomonas alcaligenes
-
 oligo(3-hydroxalkanoate)
-
 ?
3.1.1.76 poly(3-hydroxyalkanoate) + H2O Pseudomonas alcaligenes LB19
-
 oligo(3-hydroxyalkanoate) + 3-hydroxyalkanoate
-
 ?
3.1.1.76 poly(3-hydroxyalkanoate) + H2O Pseudomonas alcaligenes M4-7
-
 oligo(3-hydroxalkanoate)
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.76 Pseudomonas alcaligenes Q6UFW4 gene phaZ, precursor; gene phaZ
-
3.1.1.76 Pseudomonas alcaligenes Q84C08 gene phaZ, precursor; gene phaZ
-
3.1.1.76 Pseudomonas alcaligenes LB19 Q6UFW4 gene phaZ, precursor; gene phaZ
-
3.1.1.76 Pseudomonas alcaligenes M4-7 Q84C08 gene phaZ, precursor; gene phaZ
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.76 from strain M4-7 Pseudomonas alcaligenes

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.1.76 [oxycarbonyl[(R)-2-pentylethylene]]n + H2O = [oxycarbonyl[(R)-2-pentylethylene]]m + [oxycarbonyl[(R)-2-pentylethylene]]g Asn111 and Ser110 function as oxyanions important in transition-state stabilization of the hydrolytic reaction Pseudomonas alcaligenes
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.76 4-nitrophenyl octanoate + H2O
-
 Pseudomonas alcaligenes 4-nitrophenol + octanoate
-
 ?
3.1.1.76 4-nitrophenyl octanoate + H2O
-
 Pseudomonas alcaligenes LB19 4-nitrophenol + octanoate
-
 ?
3.1.1.76 4-nitrophenyl octanoate + H2O
-
 Pseudomonas alcaligenes M4-7 4-nitrophenol + octanoate
-
 ?
3.1.1.76 poly(3-hydroxyalkanoate) + H2O
-
 Pseudomonas alcaligenes oligo(3-hydroxyalkanoate) + 3-hydroxyalkanoate
-
 ?
3.1.1.76 poly(3-hydroxyalkanoate) + H2O
-
 Pseudomonas alcaligenes LB19 oligo(3-hydroxyalkanoate) + 3-hydroxyalkanoate
-
 ?
3.1.1.76 poly(3-hydroxyalkanoate) + H2O
-
 Pseudomonas alcaligenes oligo(3-hydroxalkanoate)
-
 ?
3.1.1.76 poly(3-hydroxyalkanoate) + H2O
-
 Pseudomonas alcaligenes M4-7 oligo(3-hydroxalkanoate)
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.76 ? x * 28000, SDS-PAGE Pseudomonas alcaligenes
3.1.1.76 ? x * 30323, amino acid sequence calculation Pseudomonas alcaligenes
3.1.1.76 More the enzyme contains a lipase box Pseudomonas alcaligenes
3.1.1.76 More the enzyme possesses a catalytic domain in the N-terminus, and a separate substrate binding domain in the C-terminus, the enzyme contains a lipase box Pseudomonas alcaligenes

Synonyms

EC Number Synonyms Comment Organism
3.1.1.76 MCL-PHA depolymerase
-
 Pseudomonas alcaligenes
3.1.1.76 medium-chain-length poly(3-hydroxyalkanoate) depolymerase
-
 Pseudomonas alcaligenes
3.1.1.76 PhaZ
-
 Pseudomonas alcaligenes

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.76 35
-
-
 Pseudomonas alcaligenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.76 8.5
-
-
 Pseudomonas alcaligenes
3.1.1.76 9
-
-
 Pseudomonas alcaligenes

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.76 Pseudomonas alcaligenes amino acid sequence calculation
-
 5.7
3.1.1.76 Pseudomonas alcaligenes amino acid sequence calculation
-
 5.9