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Literature summary extracted from

  • Pan, Q.; Zou, K.; Peng, C.; Wang, X.; Zhang, D.
    Purification, biochemical and immunological characterization of acid invertases from apple fruit (2005), J. Integr. Plant Biol., 47, 50-59.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.26 isozymes SAI and CWI, DNA and amino acid sequence determinations and analysis Malus domestica

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.26 additional information
-
additional information kinetics, isozymes SAI and CWI, isozyme Sai has a lower KM for sucrose and a higher KM for raffinose compared to isozyme CWI Malus domestica

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.26 cell wall cell wall-bound ivertase isozyme, CWI Malus domestica 5618
-
3.2.1.26 soluble soluble acid invertase isozyme, SAI Malus domestica
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.26 50000
-
4 * 50000, isozymes SAI and CWI, SDS-PAGE Malus domestica
3.2.1.26 220000
-
isozymes SAI and CWI Malus domestica

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.26 raffinose + H2O Malus domestica
-
D-fructose + melibiose
-
ir
3.2.1.26 sucrose + H2O Malus domestica
-
D-glucose + D-fructose
-
ir

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.26 Malus domestica
-
two isozymes from cell wall and soluble fraction
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.26 native isozymes soluble acid invertase SAI and cell wall-bound invertase CWI from fruits to homogeneity Malus domestica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.26 fruit
-
Malus domestica
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.26 additional information substrate specificity of isozymes SAI and CWI, no activity with stachyose of isozymes SAI and CWI Malus domestica ?
-
?
3.2.1.26 raffinose + H2O
-
Malus domestica D-fructose + melibiose
-
ir
3.2.1.26 sucrose + H2O
-
Malus domestica D-glucose + D-fructose
-
ir

Subunits

EC Number Subunits Comment Organism
3.2.1.26 tetramer 4 * 50000, isozymes SAI and CWI, SDS-PAGE Malus domestica

Synonyms

EC Number Synonyms Comment Organism
3.2.1.26 cell wall-bound ivertase
-
Malus domestica
3.2.1.26 CWI
-
Malus domestica
3.2.1.26 invertase
-
Malus domestica
3.2.1.26 SAI
-
Malus domestica
3.2.1.26 soluble acid invertase
-
Malus domestica