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Literature summary extracted from

  • Zanoelo, F.F.; Polizeli, M.L.; Terenzi, H.F.; Jorge, J.A.
    Purification and biochemical properties of a thermostable xylose-tolerant beta-D-xylosidase from Scytalidium thermophilum (2004), J. Ind. Microbiol. Biotechnol., 31, 170-176.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.37 Ca2+ strong activation, and has a slight thermostabilizing effect Mycothermus thermophilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.37 Ag+ complete inhibition at 1 mM Mycothermus thermophilus
3.2.1.37 EDTA 20% inhibition at 1 mM Mycothermus thermophilus
3.2.1.37 Hg2+ 91% inhibition at 1 mM Mycothermus thermophilus
3.2.1.37 additional information the enzyme is very tolerant to inhibition by D-xylose and is not affected up to 200 mM D-xylose Mycothermus thermophilus
3.2.1.37 Zn2+ 22% inhibition at 1 mM Mycothermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.37 0.5
-
 4-nitrophenyl-beta-D-glucopyranoside pH 5.0, 60°C Mycothermus thermophilus
3.2.1.37 1.3
-
 4-nitrophenyl-beta-D-xylopyranoside pH 5.0, 60°C Mycothermus thermophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.37 additional information the enzyme is not affected by Mg2+, Al3+, Co2+, Mn2+, Cu2+, K+, Ba2+, and Fe2+ Mycothermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.37 Mycothermus thermophilus
-
-
-
3.2.1.37 Mycothermus thermophilus 77.8
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.37 native enzyme 125fold from mycelium by heat treatment at 50°C, ammonium sulfate fractionation, gel filtration, and ion exchange chromatography Mycothermus thermophilus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.37 mycelium
-
 Mycothermus thermophilus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.37 65
-
 purified enzyme Mycothermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.37 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Mycothermus thermophilus 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.37 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Mycothermus thermophilus 77.8 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.37 4-nitrophenyl beta-D-xylopyranoside + H2O specific for Mycothermus thermophilus 4-nitrophenol + beta-D-xylose
-
 ?
3.2.1.37 4-nitrophenyl beta-D-xylopyranoside + H2O specific for Mycothermus thermophilus 77.8 4-nitrophenol + beta-D-xylose
-
 ?
3.2.1.37 additional information specific enzyme, no activity with 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-alpha-L-arabinopyranoside, 2-nitrophenyl-beta-D-galactopyranoside, carboxymethyl-cellulose, avicel, or xylan Mycothermus thermophilus ?
-
 ?
3.2.1.37 additional information specific enzyme, no activity with 4-nitrophenyl-beta-D-galactopyranoside, 4-nitrophenyl-alpha-L-arabinopyranoside, 2-nitrophenyl-beta-D-galactopyranoside, carboxymethyl-cellulose, avicel, or xylan Mycothermus thermophilus 77.8 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.37 beta-D-xylosidase
-
 Mycothermus thermophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.37 60
-
-
 Mycothermus thermophilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.37 40 75 about 20% of maximal activity at 40°C, 50% at 75°C Mycothermus thermophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.37 additional information
-
 Ca2+ has a slight thermostabilizing effect Mycothermus thermophilus
3.2.1.37 50 60 60 min, fully stable Mycothermus thermophilus
3.2.1.37 65
-
 half-life is 11 min in absence of Ca2+, half-life is 30 min in presence of Ca2+ Mycothermus thermophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.37 5
-
-
 Mycothermus thermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.37 4.5 7.5 the activity drops sharply below pH 4.5 and above pH 7.0 Mycothermus thermophilus