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Literature summary extracted from

  • Fruci, D.; Ferracuti, S.; Limongi, M.Z.; Cunsolo, V.; Giorda, E.; Fraioli, R.; Sibilio, L.; Carroll, O.; Hattori, A.; Van Endert, P.M.; Giacomini, P.
    Expression of endoplasmic reticulum aminopeptidases in EBV-B cell lines from healthy donors and in leukemia/lymphoma, carcinoma, and melanoma cell lines (2006), J. Immunol., 176, 4869-4879.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.1 isozyme ERAP1, DNA and amino acid sequence determination and analysis, expression analysis in EBV-B and tumor cell lines, recombinant expression in HeLa cells Homo sapiens
3.4.11.1 isozyme ERAP2, DNA and amino acid sequence determination and analysis, expression analysis in EBV-B and tumor cell lines, recombinant expression in HeLa cells Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.1 endoplasmic reticulum
-
 Homo sapiens 5783
-
3.4.11.1 microsome
-
 Homo sapiens
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.1 115000
-
 x * 115000, ERAP1, SDS-PAGE Homo sapiens
3.4.11.1 115000
-
 x * 115000, ERAP2, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.11.1 additional information Homo sapiens the enzyme is involved in peptide trimming, e.g. for the generation of most HLA class I-binding peptide, in the endoplasmic reticulum in concert with other aminopeptidases, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.1 Homo sapiens
-
 isozyme ERAP1
-
3.4.11.1 Homo sapiens
-
 isozyme ERAP2
-
3.4.11.1 Homo sapiens Q6P179 two isozyme ERAP2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.1 by protein G affinity chromatography Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.1 1102 cell melanoma cell line Homo sapiens
-
3.4.11.1 1182 cell melanoma cell line Homo sapiens
-
3.4.11.1 1290-2 cell melanoma cell line Homo sapiens
-
3.4.11.1 1704 cell melanoma cell line Homo sapiens
-
3.4.11.1 501 cell melanoma cell line Homo sapiens
-
3.4.11.1 A-431 cell
-
 Homo sapiens
-
3.4.11.1 A-549 cell
-
 Homo sapiens
-
3.4.11.1 B-lymphoblastoid cell line 15 EBV-B lymphoblastoid cell lines, overview Homo sapiens
-
3.4.11.1 BT20 cell
-
 Homo sapiens
-
3.4.11.1 Calu1 cell
-
 Homo sapiens
-
3.4.11.1 Colo38 cell melanoma cell line Homo sapiens
-
3.4.11.1 DAUDI cell
-
 Homo sapiens
-
3.4.11.1 EBV-B cell 15 lymphoblastoid cell lines, overview Homo sapiens
-
3.4.11.1 FO-1 cell melanoma cell line Homo sapiens
-
3.4.11.1 H494 cell
-
 Homo sapiens
-
3.4.11.1 HO-1 cell melanoma cell line Homo sapiens
-
3.4.11.1 HT-29 cell
-
 Homo sapiens
-
3.4.11.1 IR-8 cell melanoma cell line Homo sapiens
-
3.4.11.1 JAR cell
-
 Homo sapiens
-
3.4.11.1 JEG-3 cell
-
 Homo sapiens
-
3.4.11.1 K-562 cell
-
 Homo sapiens
-
3.4.11.1 KJ29 cell
-
 Homo sapiens
-
3.4.11.1 LoVo cell
-
 Homo sapiens
-
3.4.11.1 LS174T cell
-
 Homo sapiens
-
3.4.11.1 M10 cell melanoma cell line Homo sapiens
-
3.4.11.1 M14 cell melanoma cell line Homo sapiens
-
3.4.11.1 MCF-7 cell
-
 Homo sapiens
-
3.4.11.1 MEL249 cell melanoma cell line Homo sapiens
-
3.4.11.1 MOLT-4 cell
-
 Homo sapiens
-
3.4.11.1 additional information quantitative analysis of isozyme expression in EBV-B and diverse cancer cell lines, overview Homo sapiens
-
3.4.11.1 MRN-1 cell melanoma cell line Homo sapiens
-
3.4.11.1 SK-BR3 cell
-
 Homo sapiens
-
3.4.11.1 SK-MEL37 cell melanoma cell line Homo sapiens
-
3.4.11.1 SK-MEL93 cell melanoma cell line Homo sapiens
-
3.4.11.1 SP6.5 cell
-
 Homo sapiens
-
3.4.11.1 STP-1 cell melanoma cell line Homo sapiens
-
3.4.11.1 T24 cell
-
 Homo sapiens
-
3.4.11.1 U-937 cell
-
 Homo sapiens
-
3.4.11.1 WI-L2 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.1 L-arginyl-7-amido-4-methylcoumarin + H2O ERAP2 preferentially hydrolyzes Arg-aminomethylcoumarin and Lys-aminomethylcoumarin Homo sapiens L-arginine + 7-amino-4-methylcoumarin
-
 ?
3.4.11.1 L-leucyl-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens L-leucine + 7-amino-4-methylcoumarin
-
 ?
3.4.11.1 L-leucyl-7-amido-4-methylcoumarin + H2O ERAP1 preferentially hydrolyzes Leu-aminoacyl-aminomethylcoumarin Homo sapiens L-leucine + 7-amino-4-methylcoumarin
-
 ?
3.4.11.1 L-lysyl-7-amido-4-methylcoumarin + H2O ERAP2 preferentially hydrolyzes Arg-aminomethylcoumarin and Lys-aminomethylcoumarin Homo sapiens L-lysine + 7-amino-4-methylcoumarin
-
 ?
3.4.11.1 leucyl-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens leucine + 7-amino-4-methylcoumarin
-
 ?
3.4.11.1 additional information the enzyme is involved in peptide trimming, e.g. for the generation of most HLA class I-binding peptide, in the endoplasmic reticulum in concert with other aminopeptidases, overview Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.1 ? x * 115000, ERAP1, SDS-PAGE Homo sapiens
3.4.11.1 ? x * 115000, ERAP2, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.11.1 endoplasmic reticulum aminopeptidase
-
 Homo sapiens
3.4.11.1 ERAP
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.1 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.1 7.5
-
 assay at Homo sapiens