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Literature summary extracted from
- Polymorphism and modulation of cell wall esterase enzyme activities in the chicory root during the growing season (2006), J. Exp. Bot., 57, 81-89.
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.1.6 | cell wall | - |
Cichorium intybus | 5618 | - |
| 3.1.1.11 | cell wall | - |
Cichorium intybus | 5618 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.6 | 35000 | - |
x * 35000, x * 50000, and x * 65000, SDS-PAGE of enzyme isoforms | Cichorium intybus |
| 3.1.1.6 | 50000 | - |
x * 35000, x * 50000, and x * 65000, SDS-PAGE of enzyme isoforms | Cichorium intybus |
| 3.1.1.6 | 65000 | - |
x * 35000, x * 50000, and x * 65000, SDS-PAGE of enzyme isoforms | Cichorium intybus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.11 | pectin + H2O | Cichorium intybus | involved in the regulation of the cell wall rigidity, important role in plant growth and differentiation, enzyme activity in Nausica variety is correlated with ambient temperature | methanol + pectate | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.6 | Cichorium intybus | - |
cv. Nausica and Arancha | - |
| 3.1.1.11 | Cichorium intybus | - |
cv. Nausica and Arancha, highest enzyme activity at the beginning of the season | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.1.6 | root | - |
Cichorium intybus | - |
| 3.1.1.11 | root | - |
Cichorium intybus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.6 | additional information | - |
isoforms with pI-values 4.2 and 5.0 are the most active ones in all samples | Cichorium intybus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.6 | triacetine + H2O | - |
Cichorium intybus | ? | - |
? | |
| 3.1.1.11 | pectin + H2O | citrus pectin | Cichorium intybus | methanol + pectate | - |
? | |
| 3.1.1.11 | pectin + H2O | involved in the regulation of the cell wall rigidity, important role in plant growth and differentiation, enzyme activity in Nausica variety is correlated with ambient temperature | Cichorium intybus | methanol + pectate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.6 | ? | x * 35000, x * 50000, and x * 65000, SDS-PAGE of enzyme isoforms | Cichorium intybus |
| 3.1.1.11 | ? | three different isoforms with molecular weights of 75000, 83000, and 91000 detected in SDS-PAGE | Cichorium intybus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.11 | pectin methylesterase | - |
Cichorium intybus |
| 3.1.1.11 | PME | - |
Cichorium intybus |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.1.1.6 | Cichorium intybus | all isoforms isolated have pI-values between 4.1 and 5.2 | - |
additional information |
| 3.1.1.11 | Cichorium intybus | acidic and alkaline isoforms detected in both varieties | - |
additional information |
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Release 2023.1 (January 2023)
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