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Literature summary extracted from
- Purification and characterization of carbonic anhydrase from bovine stomach and effects of some known inhibitors on enzyme activity (2005), J. Enzyme Inhib. Med. Chem., 20, 75-80.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.1 | KSCN | uncompetitive inhibition of outer peripheral, cytosolic, inner peripheral and integral isoenzyme | Bos taurus |  |
| 4.2.1.1 | NaN3 | uncompetitive inhibition of outer peripheral, cytosolic, inner peripheral and integral isoenzyme | Bos taurus | |
| 4.2.1.1 | sulfanilamide | uncompetitive inhibition of outer peripheral, cytosolic, inner peripheral and integral isoenzyme | Bos taurus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.1 | 0.541 | - |
4-nitrophenyl acetate | pH 7.4, 20°C, cytosolic isoenzyme | Bos taurus | |
| 4.2.1.1 | 0.625 | - |
4-nitrophenyl acetate | pH 7.4, 20°C, outer peripheral isoenzyme | Bos taurus | |
| 4.2.1.1 | 0.785 | - |
4-nitrophenyl acetate | pH 7.4, 20°C, inner peripheral isoenzyme | Bos taurus | |
| 4.2.1.1 | 0.862 | - |
4-nitrophenyl acetate | pH 7.4, 20°C, integral isoenzyme | Bos taurus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 4.2.1.1 | cytosol | - |
Bos taurus | 5829 | - |
| 4.2.1.1 | additional information | outer peripheral, inner peripheral or integral localization | Bos taurus | - |
- |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.1 | Bos taurus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.1 | - |
Bos taurus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.2.1.1 | stomach | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.1 | additional information | - |
- |
Bos taurus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.1 | 4-nitrophenyl acetate + H2O | - |
Bos taurus | 4-nitrophenol + acetate | - |
? | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.1 | 0.000094 | - |
KSCN | pH 7.4, 20°C, inner peripheral isoenzyme | Bos taurus | |
| 4.2.1.1 | 0.000119 | - |
sulfanilamide | pH 7.4, 20°C, outer peripheral isoenzyme | Bos taurus | |
| 4.2.1.1 | 0.00013 | - |
sulfanilamide | pH 7.4, 20°C, cytosolic isoenzyme | Bos taurus | |
| 4.2.1.1 | 0.000134 | - |
KSCN | pH 7.4, 20°C, cytosolic isoenzyme | Bos taurus | |
| 4.2.1.1 | 0.00015 | - |
NaN3 | pH 7.4, 20°C, integral isoenzyme | Bos taurus | |
| 4.2.1.1 | 0.000152 | - |
KSCN | pH 7.4, 20°C, outer peripheral isoenzyme | Bos taurus | |
| 4.2.1.1 | 0.000165 | - |
KSCN | pH 7.4, 20°C, integral isoenzyme | Bos taurus | |
| 4.2.1.1 | 0.00023 | - |
sulfanilamide | pH 7.4, 20°C, inner peripheral isoenzyme | Bos taurus | |
| 4.2.1.1 | 0.000285 | - |
sulfanilamide | pH 7.4, 20°C, integral isoenzyme | Bos taurus | |
| 4.2.1.1 | 0.00033 | - |
NaN3 | pH 7.4, 20°C, cytosolic isoenzyme | Bos taurus | |
| 4.2.1.1 | 0.000596 | - |
NaN3 | pH 7.4, 20°C, inner peripheral isoenzyme | Bos taurus | |
| 4.2.1.1 | 0.000668 | - |
NaN3 | pH 7.4, 20°C, outer peripheral isoenzyme | Bos taurus | |
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