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Literature summary extracted from

  • Grace, P.B.; Teale, P.
    Purification of the crude solution from Helix pomatia for use as beta-glucuronidase and aryl sulfatase in phytoestrogen assays (2006), J. Chromatogr. B, 832, 158-161.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.6.1 synthesis purification method for removing phytoestrogens and related compounds without affecting the enzyme activity. Use of solid phase extraction on a polymeric resin for purification Helix pomatia
3.2.1.31 analysis the enzyme is useful in analysis of phytoestrogens and related compounds in human biofluids, e.g. urine Helix pomatia
3.2.1.31 diagnostics the enzyme is useful in analysis of phytoestrogens and related compounds in human biofluids, e.g. urine Helix pomatia

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.31 extracellular digestive juice, composition of the digestive juice containing enzyme and phytoestrogen compounds, overview Helix pomatia
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Organism

EC Number Organism UniProt Comment Textmining
3.1.6.1 Helix pomatia
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 bifunctional beta-glucuronidase/aryl sulfatase
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3.2.1.31 Helix pomatia
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Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.6.1 use of solid phase extraction on a polymeric resin for purification without affecting the enzyme activity Helix pomatia
3.2.1.31 native enzyme from digestive juice by ion exchange chromatography on a resin containing a polymeric sorbent Helix pomatia

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.31 additional information
-
 activities of crude and purified enzyme from digestive juice Helix pomatia

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.31 additional information substrate specificity with flavonoids and phytoestrogens of crude and purified enzyme from digestive juice, overview Helix pomatia ?
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 ?