Any feedback?
Literature summary extracted from
- Purification, characterization, and synergistic action of phytate-resistant alpha-amylase and alpha-glucosidase from Geobacillus thermodenitrificans HRO10 (2006), J. Biotechnol., 125, 27-38.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.1 | additional information | no inhibition by phytate at up to 10 mM | Geobacillus thermodenitrificans | |
| 3.2.1.20 | 4-chloromercuribenzoate | complete inhibition at 2 mM | Geobacillus thermodenitrificans |  |
| 3.2.1.20 | EDTA | 22% inhibition at 10 mM | Geobacillus thermodenitrificans | |
| 3.2.1.20 | iodoacetic acid | 23% inhibition at 10 mM | Geobacillus thermodenitrificans | |
| 3.2.1.20 | additional information | no inhibition by phytate | Geobacillus thermodenitrificans |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.1 | 3.05 | - |
soluble starch | pH 5.5, 80°C | Geobacillus thermodenitrificans | |
| 3.2.1.20 | additional information | - |
additional information | the enzyme exhibits Michaelis-Menten kinetics | Geobacillus thermodenitrificans | |
| 3.2.1.20 | 17 | - |
maltose | pH 7.0, 55°C | Geobacillus thermodenitrificans | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.1 | extracellular | the enzyme is secreted | Geobacillus thermodenitrificans | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.1 | Ca2+ | stabilizes the enzyme | Geobacillus thermodenitrificans | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 58000 | - |
x * 58000, SDS-PAGE | Geobacillus thermodenitrificans |
| 3.2.1.20 | 45000 | - |
x * 45000, SDS-PAGE | Geobacillus thermodenitrificans |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.20 | maltose + H2O | Geobacillus thermodenitrificans | - |
alpha-D-glucose + D-glucose | - |
? | |
| 3.2.1.20 | maltose + H2O | Geobacillus thermodenitrificans HRO10 | - |
alpha-D-glucose + D-glucose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.1 | Geobacillus thermodenitrificans | - |
- |
- |
| 3.2.1.1 | Geobacillus thermodenitrificans HRO10 | - |
- |
- |
| 3.2.1.20 | Geobacillus thermodenitrificans | - |
- |
- |
| 3.2.1.20 | Geobacillus thermodenitrificans HRO10 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.1 | native extracellular enzyme 13.6fold to homogeneity in a multistep procedure | Geobacillus thermodenitrificans |
| 3.2.1.20 | native enzyme 25.4fold to homogeneity by ultrafiltration, chromatofocusing, and gel filtration | Geobacillus thermodenitrificans |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.1 | soluble starch + H2O | - |
Geobacillus thermodenitrificans | ? | - |
? | |
| 3.2.1.1 | soluble starch + H2O | - |
Geobacillus thermodenitrificans HRO10 | ? | - |
? | |
| 3.2.1.1 | starch + H2O | soluble starch from potato | Geobacillus thermodenitrificans | malto-oligosaccharides | - |
? | |
| 3.2.1.1 | starch + H2O | soluble starch from potato | Geobacillus thermodenitrificans HRO10 | malto-oligosaccharides | - |
? | |
| 3.2.1.20 | maltose + H2O | - |
Geobacillus thermodenitrificans | alpha-D-glucose + D-glucose | - |
? | |
| 3.2.1.20 | maltose + H2O | - |
Geobacillus thermodenitrificans HRO10 | alpha-D-glucose + D-glucose | - |
? | |
| 3.2.1.20 | additional information | strain HRO10 alpha-amylase and alpha-glucosidase show an excellent synergistic action and do not reveal any transglycosylation catalyzed reaction by the alpha-glucosidase | Geobacillus thermodenitrificans | ? | - |
? | |
| 3.2.1.20 | additional information | strain HRO10 alpha-amylase and alpha-glucosidase show an excellent synergistic action and do not reveal any transglycosylation catalyzed reaction by the alpha-glucosidase | Geobacillus thermodenitrificans HRO10 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.1 | ? | x * 58000, SDS-PAGE | Geobacillus thermodenitrificans |
| 3.2.1.20 | ? | x * 45000, SDS-PAGE | Geobacillus thermodenitrificans |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 80 | - |
- |
Geobacillus thermodenitrificans |
| 3.2.1.20 | 55 | - |
- |
Geobacillus thermodenitrificans |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.20 | 35 | 75 | 30% of maximal activity at 35°C, 80% of maximal activity at 45-60°C, inactive above 75°C | Geobacillus thermodenitrificans |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 70 | - |
1 h, in presence of Ca2+, 92% remaining activity | Geobacillus thermodenitrificans |
| 3.2.1.20 | 60 | - |
purified enzyme, 30 min, 70% activity remaining | Geobacillus thermodenitrificans |
| 3.2.1.20 | 70 | - |
purified enzyme, 20 min, 3.7% activity remaining | Geobacillus thermodenitrificans |
| 3.2.1.20 | 75 | - |
inactivation | Geobacillus thermodenitrificans |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 5.5 | - |
- |
Geobacillus thermodenitrificans |
| 3.2.1.20 | 6.5 | 7.5 | - |
Geobacillus thermodenitrificans |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
