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Literature summary extracted from

  • Nakkharat, P.; Haltrich, D.
    Purification and characterisation of an intracellular enzyme with beta-glucosidase and beta-galactosidase activity from the thermophilic fungus Talaromyces thermophilus CBS 236.58 (2006), J. Biotechnol., 123, 304-313.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 cellobiose substrate inhibition at 10 mM Thermomyces dupontii
3.2.1.21 Cu2+
-
 Thermomyces dupontii
3.2.1.21 D-fructose
-
 Thermomyces dupontii
3.2.1.21 D-galactose competitive product inhibition Thermomyces dupontii
3.2.1.21 D-glucose competitive product inhibition Thermomyces dupontii
3.2.1.21 D-xylose
-
 Thermomyces dupontii
3.2.1.21 L-arabinose
-
 Thermomyces dupontii
3.2.1.21 lactose
-
 Thermomyces dupontii
3.2.1.21 maltose
-
 Thermomyces dupontii
3.2.1.23 galactose
-
 Thermomyces dupontii
3.2.1.23 glucose
-
 Thermomyces dupontii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 additional information
-
 additional information steady-state kinetics Thermomyces dupontii
3.2.1.21 1.2
-
 2-Nitrophenyl-beta-D-glucopyranoside pH 6.5, 40°C Thermomyces dupontii
3.2.1.21 5.7
-
 cellobiose pH 6.5, 40°C Thermomyces dupontii
3.2.1.21 24
-
 2-nitrophenyl-beta-D-galactopyranoside pH 6.5, 40°C Thermomyces dupontii

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.21 intracellular
-
 Thermomyces dupontii 5622
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.21 Ca2+ activates Thermomyces dupontii
3.2.1.21 Co2+ activates Thermomyces dupontii
3.2.1.21 Fe2+ activates Thermomyces dupontii
3.2.1.21 K+ activates Thermomyces dupontii
3.2.1.21 Mg2+ activates Thermomyces dupontii
3.2.1.21 Mn2+ activates Thermomyces dupontii
3.2.1.21 Na+ activates Thermomyces dupontii
3.2.1.21 Zn2+ activates Thermomyces dupontii
3.2.1.23 K+ activates Thermomyces dupontii
3.2.1.23 Mg2+ activates Thermomyces dupontii
3.2.1.23 Na+ activates Thermomyces dupontii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 49000
-
 gel filtration Thermomyces dupontii
3.2.1.21 50000
-
 1 * 50000, SDS-PAGE Thermomyces dupontii
3.2.1.23 50000
-
 1 * 50000, SDS-PAGE Thermomyces dupontii

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Thermomyces dupontii
-
-
-
3.2.1.21 Thermomyces dupontii CBS 236.58
-
-
-
3.2.1.23 Thermomyces dupontii
-
 CBS 236.58
-
3.2.1.23 Thermomyces dupontii CBS 236.58
-
 CBS 236.58
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 native enzyme 10.2fold to homogeneity by anion exchange chromatography, ultrafiltration, and affinity chromatography using 4-aminobenzyl, 1-thio-beta-D-galactopyranoside Thermomyces dupontii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 139
-
 purified enzyme Thermomyces dupontii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Thermomyces dupontii 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.21 2-nitrophenyl beta-D-galactopyranoside + H2O
-
 Thermomyces dupontii CBS 236.58 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.21 2-nitrophenyl beta-D-glucopyranoside + H2O
-
 Thermomyces dupontii 2-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 2-nitrophenyl beta-D-glucopyranoside + H2O
-
 Thermomyces dupontii CBS 236.58 2-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Thermomyces dupontii 2 beta-D-glucose
-
 ?
3.2.1.21 additional information the enzyme shows significant transglucosylation activity, resulting in formation of e.g. galactooligosaccharides with lactose as acceptor, and is active with galactosides, overview Thermomyces dupontii ?
-
 ?
3.2.1.21 additional information the enzyme shows significant transglucosylation activity, resulting in formation of e.g. galactooligosaccharides with lactose as acceptor, and is active with galactosides, overview Thermomyces dupontii CBS 236.58 ?
-
 ?
3.2.1.23 lactose + H2O
-
 Thermomyces dupontii D-glucose + D-galactose
-
 ?
3.2.1.23 lactose + H2O
-
 Thermomyces dupontii CBS 236.58 D-glucose + D-galactose
-
 ?
3.2.1.23 additional information the enzyme is active with both glucosides such as cellobiose and galactosides including lactose. Based on the catalytic efficiencies determined glucosides are preferred substrates. In addition to its hydrolytic activity, the enzyme shows a significant transferase activity which results in formation of galacto-oligosaccharides Thermomyces dupontii ?
-
 ?
3.2.1.23 additional information the enzyme is active with both glucosides such as cellobiose and galactosides including lactose. Based on the catalytic efficiencies determined glucosides are preferred substrates. In addition to its hydrolytic activity, the enzyme shows a significant transferase activity which results in formation of galacto-oligosaccharides Thermomyces dupontii CBS 236.58 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 monomer 1 * 50000, SDS-PAGE Thermomyces dupontii
3.2.1.23 monomer 1 * 50000, SDS-PAGE Thermomyces dupontii

Synonyms

EC Number Synonyms Comment Organism
3.2.1.21 BGL1
-
 Thermomyces dupontii
3.2.1.23 BGL1
-
 Thermomyces dupontii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 50
-
-
 Thermomyces dupontii
3.2.1.23 50
-
-
 Thermomyces dupontii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.21 167
-
 cellobiose pH 6.5, 40°C Thermomyces dupontii
3.2.1.21 304
-
 2-nitrophenyl-beta-D-galactopyranoside pH 6.5, 40°C Thermomyces dupontii
3.2.1.21 308
-
 2-Nitrophenyl-beta-D-glucopyranoside pH 6.5, 40°C Thermomyces dupontii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 5.5 6
-
 Thermomyces dupontii
3.2.1.23 5.5 6
-
 Thermomyces dupontii

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.21 66
-
 D-glucose versus 2-nitrophenyl-beta-D-galactopyranoside, pH 6.5, 40°C Thermomyces dupontii
3.2.1.21 370
-
 D-galactose versus 2-nitrophenyl-beta-D-galactopyranoside, pH 6.5, 40°C Thermomyces dupontii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.21 Thermomyces dupontii isoelectric focusing 4.6 4.5
3.2.1.23 Thermomyces dupontii
-
 4.6 4.5