Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Klu-Santner, B.G.; Schnitzhofer, W.; et.al.
    Purification and characterization of a new bioscouring pectate lyase from Bacillus pumilus BK2 (2006), J. Biotechnol., 121, 390-401.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.2.2.2 biotechnology the enzyme can be used for bioscouring of cotton Bacillus pumilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.2 Co2+ 0.38 mM, 50% inhibition Bacillus pumilus
4.2.2.2 EDTA 0.09 mM, 50% inhibition Bacillus pumilus
4.2.2.2 Zn2+ 2.8 mM, 50% inhibition Bacillus pumilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.2.2 additional information
-
 additional information KM-value for polygalacturonate is 0.24 g/l, at pH 7.8, 50°C. KM-value is 0.24 g/l for not esterified pectin, 0.36 g/l for pectin with 5.3% esterification, 0.35 g/l for pectin with 29.5% esterification, 1.34 g/l for pectin with 51.4% esterification Bacillus pumilus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.2.2 extracellular
-
 Bacillus pumilus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.2 additional information enzyme does not require Ca2+ Bacillus pumilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.2 37300
-
 x * 37300, SDS-PAGE Bacillus pumilus

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.2 Bacillus pumilus
-
-
-
4.2.2.2 Bacillus pumilus BK2
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.2
-
 Bacillus pumilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.2 esterified pectin affinity shows a maximum for intermediate esterified pectins and decreases over a value of 50% of esterification. The best substrate is 29.5% methylated pectin Bacillus pumilus unsaturated tetragalacturonic acid
-
 ?
4.2.2.2 esterified pectin affinity shows a maximum for intermediate esterified pectins and decreases over a value of 50% of esterification. The best substrate is 29.5% methylated pectin Bacillus pumilus BK2 unsaturated tetragalacturonic acid
-
 ?
4.2.2.2 polygalacturonate the enzyme cleaves polygalacturonic acid via a beta-elimination mechanism Bacillus pumilus unsaturated tetragalacturonic acid
-
 ?
4.2.2.2 polygalacturonate the enzyme cleaves polygalacturonic acid via a beta-elimination mechanism Bacillus pumilus BK2 unsaturated tetragalacturonic acid
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.2.2 ? x * 37300, SDS-PAGE Bacillus pumilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.2 70
-
-
 Bacillus pumilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.2 30
-
 half-life: more than 75 h Bacillus pumilus
4.2.2.2 80
-
 half-life at pH 8.0 and 7.0 is 1.2 min, half-life at pH 9.0 is 0.8 min Bacillus pumilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.2 8.5
-
-
 Bacillus pumilus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.2.2 Bacillus pumilus 2D PAGE, pH-range 3-10
-
 8.5