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Literature summary extracted from

  • Baciu, I.E.; Joerdening, H.J.; Seibel, J.; Buchholz, K.
    Investigations of the transfructosylation reaction by fructosyltransferase from B. subtilis NCIMB 11871 for the synthesis of the sucrose analogue galactosyl-fructoside (2004), J. Biotechnol., 116, 347-357.
    View publication on PubMed

General Stability

EC Number General Stability Organism
4.2.2.16 the enzyme is immobilized on Eupergit C250 L and Trisospor-amino without loss of enzymatic activity Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.162 Bacillus subtilis
-
 NCIMB 11871
-
2.4.1.162 Bacillus subtilis 168
-
 NCIMB 11871
-
4.2.2.16 Bacillus subtilis
-
 NCIMB 11871
-
4.2.2.16 Bacillus subtilis 168
-
 NCIMB 11871
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.162 sucrose + D-galactose
-
 Bacillus subtilis galactosylfructoside + D-glucose
-
 ?
2.4.1.162 sucrose + D-galactose
-
 Bacillus subtilis 168 galactosylfructoside + D-glucose
-
 ?
4.2.2.16 raffinose + D-galactopyranose transfer of the beta-fructofuranosyl residue from donor substrate to the 1-C of the acceptor galactopyranose, equilibrium towards galactosyl-fructoside Bacillus subtilis ?
-
 r
4.2.2.16 raffinose + D-galactopyranose transfer of the beta-fructofuranosyl residue from donor substrate to the 1-C of the acceptor galactopyranose, equilibrium towards galactosyl-fructoside Bacillus subtilis 168 ?
-
 r
4.2.2.16 stachyose + D-galactopyranose transfer of the beta-fructofuranosyl residue from donor substrate to the 1-C of the acceptor galactopyranose, equilibrium towards galactosyl-fructoside Bacillus subtilis ?
-
 r
4.2.2.16 stachyose + D-galactopyranose transfer of the beta-fructofuranosyl residue from donor substrate to the 1-C of the acceptor galactopyranose, equilibrium towards galactosyl-fructoside Bacillus subtilis 168 ?
-
 r
4.2.2.16 sucrose + D-galactopyranose transfer of the beta-fructofuranosyl residue from donor substrate to the 1-C of the acceptor galactopyranose, equilibrium towards galactosyl-fructoside Bacillus subtilis ?
-
 r
4.2.2.16 sucrose + D-galactopyranose transfer of the beta-fructofuranosyl residue from donor substrate to the 1-C of the acceptor galactopyranose, equilibrium towards galactosyl-fructoside Bacillus subtilis 168 ?
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.2.2.16 FTF
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.162 50
-
-
 Bacillus subtilis
4.2.2.16 50
-
-
 Bacillus subtilis