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Literature summary extracted from

  • MMorimoto, N.; Yasukawa, Y.; Watanabe, K.; Unno, T.; Ito, H.; Matsui, H.
    Cloning and heterologous expression of a glucodextranase gene from Arthrobacter globiformis I42, and experimental evidence for the catalytic diad of the recombinant enzyme (2004), J. Biosci. Bioeng., 97, 127-130.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.70 expression in Escherichia coli Arthrobacter globiformis

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.70 E458Q mutant enzyme shows 0.0005% of the wild-type activity Arthrobacter globiformis
3.2.1.70 E656Q mutant enzyme shows 0.0003% of the wild-type activity Arthrobacter globiformis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.70 additional information
-
 additional information KM-value for dextran is 0.031 mg/ml Arthrobacter globiformis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.70 109135
-
 x * 109135, calculated from sequence Arthrobacter globiformis
3.2.1.70 120000
-
 x * 120000, SDS-PAGE Arthrobacter globiformis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.70 Arthrobacter globiformis
-
-
-
3.2.1.70 Arthrobacter globiformis I42
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.70 wild-type enzyme and mutant enzymes E458Q and E656Q Arthrobacter globiformis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.70 dextran + H2O
-
 Arthrobacter globiformis glucose
-
 ?
3.2.1.70 dextran + H2O
-
 Arthrobacter globiformis I42 glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.70 ? x * 120000, SDS-PAGE Arthrobacter globiformis
3.2.1.70 ? x * 109135, calculated from sequence Arthrobacter globiformis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.70 45
-
-
 Arthrobacter globiformis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.70 55
-
 10 min, less than 20% loss of activity Arthrobacter globiformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.70 6
-
-
 Arthrobacter globiformis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.70 5.5 7.4 more than 80% of maximal activity betwenn pH 5.5 and pH 7.4 Arthrobacter globiformis