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Literature summary extracted from

  • D'Souza, J.S.; Johri, M.M.
    Purification and characterization of a Ca2+-dependent/calmodulin-stimulated protein kinase from moss chloronema cells (2003), J. Biosci., 28, 223-233.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.17 Calmodulin dependent on, activates at 3-1000 nM Funaria hygrometrica
2.7.11.17 additional information the enzyme is activated by strictly Ca2+-dependent autophosphorylation Funaria hygrometrica

General Stability

EC Number General Stability Organism
2.7.11.17 activity of the purified enzyme is completely lost upon a freeze-thaw cycle Funaria hygrometrica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.17 Ca2+ dependent on, activates, optimal at 0.05 mM Funaria hygrometrica
2.7.11.17 Mg2+
-
Funaria hygrometrica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.11.17 70000
-
x * 70000, SDS-PAGE Funaria hygrometrica

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
2.7.11.17 n-propanol for extraction and as additive at 5% to the activity assay buffer Funaria hygrometrica

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.17 Funaria hygrometrica
-
a moss
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.17 additional information the enzyme performs activating strictly Ca2+-dependent autophosphorylation which is influenced by substrates, overview Funaria hygrometrica

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.11.17 native enzyme from chloronemal cells 44fold to near homogeneity by ammoinum sulfate fractionation, DEAE-cellulose ion exchange chromatography, and calmodulin-agarose affinity chromatography in presence of CaCl2 Funaria hygrometrica

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.17 chloronema
-
Funaria hygrometrica
-
2.7.11.17 protonema chloronemal cells Funaria hygrometrica
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.11.17 0.00069
-
purified enzyme in presence of Ca2+ with substrate histone IIIS Funaria hygrometrica

Storage Stability

EC Number Storage Stability Organism
2.7.11.17 25°C, purified enzyme, increased activity at day 1 to 9, rapid loss within day 10, activity is completely lost upon a freeze-thaw cycle Funaria hygrometrica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.17 ATP + casein
-
Funaria hygrometrica ADP + phosphorylated casein
-
?
2.7.11.17 ATP + histone IIA
-
Funaria hygrometrica ADP + phosphorylated histone IIA
-
?
2.7.11.17 ATP + histone IIIS
-
Funaria hygrometrica ADP + phosphorylated histone IIIS
-
?
2.7.11.17 ATP + histone VI
-
Funaria hygrometrica ADP + phosphorylated histone VI
-
?
2.7.11.17 ATP + histone VIIS
-
Funaria hygrometrica ADP + phosphorylated histone VIIS
-
?
2.7.11.17 ATP + histone VIS
-
Funaria hygrometrica ADP + phosphorylated histone VIS
-
?
2.7.11.17 ATP + histone VS
-
Funaria hygrometrica ADP + phosphorylated histone VS
-
?
2.7.11.17 additional information the enzyme prefers lysine-rich substrates, no activity with phosvitin and bovine serum albumin, the enzyme performs strictly Ca2+-dependent autophosphorylation which is influenced by substrates, overview Funaria hygrometrica ?
-
?

Subunits

EC Number Subunits Comment Organism
2.7.11.17 ? x * 70000, SDS-PAGE Funaria hygrometrica

Synonyms

EC Number Synonyms Comment Organism
2.7.11.17 CCaMK
-
Funaria hygrometrica
2.7.11.17 PK70
-
Funaria hygrometrica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.17 25
-
assay at Funaria hygrometrica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.17 7.5
-
assay at Funaria hygrometrica

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.17 ATP
-
Funaria hygrometrica