EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.7.11.17 | Calmodulin | dependent on, activates at 3-1000 nM | Funaria hygrometrica | |
2.7.11.17 | additional information | the enzyme is activated by strictly Ca2+-dependent autophosphorylation | Funaria hygrometrica |
EC Number | General Stability | Organism |
---|---|---|
2.7.11.17 | activity of the purified enzyme is completely lost upon a freeze-thaw cycle | Funaria hygrometrica |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.11.17 | Ca2+ | dependent on, activates, optimal at 0.05 mM | Funaria hygrometrica | |
2.7.11.17 | Mg2+ | - |
Funaria hygrometrica |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.11.17 | 70000 | - |
x * 70000, SDS-PAGE | Funaria hygrometrica |
EC Number | Organic Solvent | Comment | Organism |
---|---|---|---|
2.7.11.17 | n-propanol | for extraction and as additive at 5% to the activity assay buffer | Funaria hygrometrica |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.11.17 | Funaria hygrometrica | - |
a moss | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
2.7.11.17 | additional information | the enzyme performs activating strictly Ca2+-dependent autophosphorylation which is influenced by substrates, overview | Funaria hygrometrica |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.11.17 | native enzyme from chloronemal cells 44fold to near homogeneity by ammoinum sulfate fractionation, DEAE-cellulose ion exchange chromatography, and calmodulin-agarose affinity chromatography in presence of CaCl2 | Funaria hygrometrica |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.11.17 | chloronema | - |
Funaria hygrometrica | - |
2.7.11.17 | protonema | chloronemal cells | Funaria hygrometrica | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.11.17 | 0.00069 | - |
purified enzyme in presence of Ca2+ with substrate histone IIIS | Funaria hygrometrica |
EC Number | Storage Stability | Organism |
---|---|---|
2.7.11.17 | 25°C, purified enzyme, increased activity at day 1 to 9, rapid loss within day 10, activity is completely lost upon a freeze-thaw cycle | Funaria hygrometrica |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.11.17 | ATP + casein | - |
Funaria hygrometrica | ADP + phosphorylated casein | - |
? | |
2.7.11.17 | ATP + histone IIA | - |
Funaria hygrometrica | ADP + phosphorylated histone IIA | - |
? | |
2.7.11.17 | ATP + histone IIIS | - |
Funaria hygrometrica | ADP + phosphorylated histone IIIS | - |
? | |
2.7.11.17 | ATP + histone VI | - |
Funaria hygrometrica | ADP + phosphorylated histone VI | - |
? | |
2.7.11.17 | ATP + histone VIIS | - |
Funaria hygrometrica | ADP + phosphorylated histone VIIS | - |
? | |
2.7.11.17 | ATP + histone VIS | - |
Funaria hygrometrica | ADP + phosphorylated histone VIS | - |
? | |
2.7.11.17 | ATP + histone VS | - |
Funaria hygrometrica | ADP + phosphorylated histone VS | - |
? | |
2.7.11.17 | additional information | the enzyme prefers lysine-rich substrates, no activity with phosvitin and bovine serum albumin, the enzyme performs strictly Ca2+-dependent autophosphorylation which is influenced by substrates, overview | Funaria hygrometrica | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.11.17 | ? | x * 70000, SDS-PAGE | Funaria hygrometrica |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.11.17 | CCaMK | - |
Funaria hygrometrica |
2.7.11.17 | PK70 | - |
Funaria hygrometrica |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.11.17 | 25 | - |
assay at | Funaria hygrometrica |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.11.17 | 7.5 | - |
assay at | Funaria hygrometrica |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.11.17 | ATP | - |
Funaria hygrometrica |