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Literature summary extracted from

  • Hansson, M.D.; Lindstam, M.; Hansson, M.
    Crosstalk between metal ions in Bacillus subtilis ferrochelatase (2006), J. Biol. Inorg. Chem., 11, 325-333.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.98.1.1 expression in Escherichia coli Bacillus subtilis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.98.1.1 Mg2+ is required for the formation of Bacillus subtilis ferrochelatase crystals Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
4.98.1.1 E272S more unstable than the wild-type, eliminates the stimulating effect of Mg2+ Bacillus subtilis
4.98.1.1 W230Y more unstable than the wild-type, has 7% of the wild-type activity, does not display any Zn2+-induced quenching Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.98.1.1 0.024
-
 Zn2+ wild-type, in the presence of 4 mM Mg2+, pH 7.4, room temperature Bacillus subtilis
4.98.1.1 0.05
-
 deuteroporphyrin IX mutant E272S, in the presence of 4 mM Mg2+, pH 7.4, room temperature Bacillus subtilis
4.98.1.1 0.054
-
 deuteroporphyrin IX mutant E272S, pH 7.4, room temperature Bacillus subtilis
4.98.1.1 0.055
-
 Zn2+ wild-type, pH 7.4, room temperature Bacillus subtilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.98.1.1 inclusion body the recombinant protein expressed in Escherichia coli Bacillus subtilis 16234
-
4.98.1.1 soluble in Bacillus subtilis Bacillus subtilis
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.98.1.1 Mg2+ stimulates Zn2+ insertion by Bacillus subtilis ferrochelatase Bacillus subtilis
4.98.1.1 Zn2+ quenches the fluorescence of Bacillus subtilis ferrochelatase Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.98.1.1 protoporphyrin + Fe2+ Bacillus subtilis
-
 protoheme + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.98.1.1 Bacillus subtilis P32396
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.98.1.1
-
 Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.98.1.1 deuteroporphyrin IX + Zn2+
-
 Bacillus subtilis zinc-deuteroporphyrin IX + H+
-
 ?
4.98.1.1 protoporphyrin + Fe2+
-
 Bacillus subtilis protoheme + H+
-
 ?
4.98.1.1 protoporphyrin IX + Zn2+ pH 7.4, room temperature Bacillus subtilis Zn protoporphyrin IX
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.98.1.1 ferrochelatase
-
 Bacillus subtilis