BRENDA - Enzyme Database

The Saccharomyces cerevisiae lipin homologue is a Mg2+-dependent phosphatidate phosphatase enzyme

Han, G.S.; Wu, W.I.; Carman, G.M.; J. Biol. Chem. 281, 9210-9218 (2006)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
3.1.3.4
co-overexpression of lipin with murine LPP-1 in Escherichia coli strain BL21(DE3) as His-tagged enzymes
Homo sapiens
3.1.3.4
gene PAH1, overexpression of His-tagged wild-type and mutant isozyme PAH1 in Escherichia coli strain BL21(DE3), co-expression with human lipin, subcloning in Escherichia coli strain DH5alpha
Saccharomyces cerevisiae
Engineering
EC Number
Amino acid exchange
Commentary
Organism
3.1.3.4
additional information
construction of a PAH1 mutant with reduced enzyme activity, the mutant grows more slowly and is temperature-sensitive at 37°C, mutant cells show increased phosphatidate levels compared to the wild-type cells, overview
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.4
additional information
-
additional information
steady-state kinetics, recombinant wild-type and mutant PAH1, overview
Saccharomyces cerevisiae
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
cytosol
-
Saccharomyces cerevisiae
5829
-
3.1.3.4
membrane
associated
Saccharomyces cerevisiae
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
Mg2+
dependent on
Homo sapiens
3.1.3.4
Mg2+
dependent on
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.4
lysophosphatidic acid + H2O
Homo sapiens
-
monoacylglycerol + phosphate
-
-
?
3.1.3.4
lysophosphatidic acid + H2O
Saccharomyces cerevisiae
-
monoacylglycerol + phosphate
-
-
?
3.1.3.4
phosphatidic acid + H2O
Homo sapiens
-
1,2-diacylglycerol + phosphate
-
-
?
3.1.3.4
phosphatidic acid + H2O
Saccharomyces cerevisiae
-
diacylglycerol + phosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.3.4
Homo sapiens
-
-
-
3.1.3.4
Saccharomyces cerevisiae
-
several strains, isozyme PAH1
-
Purification (Commentary)
EC Number
Commentary
Organism
3.1.3.4
recombinant His-tagged lipin from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Homo sapiens
3.1.3.4
recombinant His-tagged wild-type and mutant isozyme PAH1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.3.4
additional information
-
recombinant wild-type and mutant PAH1, assay in presence of Triton X-100 at 2 mM
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
lysophosphatidic acid + H2O
-
665748
Homo sapiens
monoacylglycerol + phosphate
-
-
-
?
3.1.3.4
lysophosphatidic acid + H2O
-
665748
Saccharomyces cerevisiae
monoacylglycerol + phosphate
-
-
-
?
3.1.3.4
additional information
the enzyme is homologous to mammalian lipin
665748
Saccharomyces cerevisiae
?
-
-
-
-
3.1.3.4
additional information
the enzyme is homologous to murine lipid phosphate phosphatase isozyme LPP-1
665748
Homo sapiens
?
-
-
-
-
3.1.3.4
phosphatidic acid + H2O
-
665748
Homo sapiens
1,2-diacylglycerol + phosphate
-
-
-
?
3.1.3.4
phosphatidic acid + H2O
-
665748
Saccharomyces cerevisiae
diacylglycerol + phosphate
-
-
-
?
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.3.4
30
-
assay at
Saccharomyces cerevisiae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.4
5
-
assay at, with substrate lysophosphatidic acid
Saccharomyces cerevisiae
3.1.3.4
7.5
-
assay at, with substrate phosphatidic acid
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.4
co-overexpression of lipin with murine LPP-1 in Escherichia coli strain BL21(DE3) as His-tagged enzymes
Homo sapiens
3.1.3.4
gene PAH1, overexpression of His-tagged wild-type and mutant isozyme PAH1 in Escherichia coli strain BL21(DE3), co-expression with human lipin, subcloning in Escherichia coli strain DH5alpha
Saccharomyces cerevisiae
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
3.1.3.4
additional information
construction of a PAH1 mutant with reduced enzyme activity, the mutant grows more slowly and is temperature-sensitive at 37°C, mutant cells show increased phosphatidate levels compared to the wild-type cells, overview
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.3.4
additional information
-
additional information
steady-state kinetics, recombinant wild-type and mutant PAH1, overview
Saccharomyces cerevisiae
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.1.3.4
cytosol
-
Saccharomyces cerevisiae
5829
-
3.1.3.4
membrane
associated
Saccharomyces cerevisiae
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.3.4
Mg2+
dependent on
Homo sapiens
3.1.3.4
Mg2+
dependent on
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3.1.3.4
lysophosphatidic acid + H2O
Homo sapiens
-
monoacylglycerol + phosphate
-
-
?
3.1.3.4
lysophosphatidic acid + H2O
Saccharomyces cerevisiae
-
monoacylglycerol + phosphate
-
-
?
3.1.3.4
phosphatidic acid + H2O
Homo sapiens
-
1,2-diacylglycerol + phosphate
-
-
?
3.1.3.4
phosphatidic acid + H2O
Saccharomyces cerevisiae
-
diacylglycerol + phosphate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.3.4
recombinant His-tagged lipin from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Homo sapiens
3.1.3.4
recombinant His-tagged wild-type and mutant isozyme PAH1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
Saccharomyces cerevisiae
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
3.1.3.4
additional information
-
recombinant wild-type and mutant PAH1, assay in presence of Triton X-100 at 2 mM
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.3.4
lysophosphatidic acid + H2O
-
665748
Homo sapiens
monoacylglycerol + phosphate
-
-
-
?
3.1.3.4
lysophosphatidic acid + H2O
-
665748
Saccharomyces cerevisiae
monoacylglycerol + phosphate
-
-
-
?
3.1.3.4
additional information
the enzyme is homologous to mammalian lipin
665748
Saccharomyces cerevisiae
?
-
-
-
-
3.1.3.4
additional information
the enzyme is homologous to murine lipid phosphate phosphatase isozyme LPP-1
665748
Homo sapiens
?
-
-
-
-
3.1.3.4
phosphatidic acid + H2O
-
665748
Homo sapiens
1,2-diacylglycerol + phosphate
-
-
-
?
3.1.3.4
phosphatidic acid + H2O
-
665748
Saccharomyces cerevisiae
diacylglycerol + phosphate
-
-
-
?
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.1.3.4
30
-
assay at
Saccharomyces cerevisiae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.3.4
5
-
assay at, with substrate lysophosphatidic acid
Saccharomyces cerevisiae
3.1.3.4
7.5
-
assay at, with substrate phosphatidic acid
Saccharomyces cerevisiae