Any feedback?
Literature summary extracted from
- Unusual structural features of the bacteriophage-associated hyaluronate lyase (hylp2) (2006), J. Biol. Chem., 281, 7143-7150.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.2.1 | overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Streptococcus pyogenes phage H10403 |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.1 | 110000 | - |
recombinant enzyme, gel filtration | Streptococcus pyogenes phage H10403 |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.2.1 | hyaluronan | Streptococcus pyogenes phage H10403 | absolute substrate specificity of the bacteriophage enzyme | hyaluronic acid oligomers | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.2.1 | Streptococcus pyogenes phage H10403 | - |
gene hylp2 | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.2.1 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) to homogeneity by ion exchange and nickel affinity chromatography, and gel filtration | Streptococcus pyogenes phage H10403 |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 4.2.2.1 | [hyaluronate]n = (4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine-[hyaluronate]n-m-1 + 2 3-(4-deoxy-beta-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine + hyaluronate | active site structure | Streptococcus pyogenes phage H10403 |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.2.1 | hyaluronan | absolute substrate specificity of the bacteriophage enzyme | Streptococcus pyogenes phage H10403 | hyaluronic acid oligomers | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.2.1 | More | three-dimensional structure analysis, unfolding process using 1-4 M guanidinium hydrochloride, effects of unfolding on the structure, transitions, kinetics, overview | Streptococcus pyogenes phage H10403 |
| 4.2.2.1 | trimer | 3 * 36000-36900, SDS-PAGE and ESI-MS | Streptococcus pyogenes phage H10403 |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.2.1 | bacteriophage-associated hyaluronate lyase | - |
Streptococcus pyogenes phage H10403 |
| 4.2.2.1 | Hylp2 | - |
Streptococcus pyogenes phage H10403 |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.1 | 25 | - |
assay at | Streptococcus pyogenes phage H10403 |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.1 | 6 | - |
assay at | Streptococcus pyogenes phage H10403 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
