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Literature summary extracted from

  • Deb, C.; Daniel, J.; Sirakova, T.D.; Abomoelak, B.; Dubey, V.S.; Kolattukudy, P.E.
    A novel lipase belonging to the hormone-sensitive lipase family induced under starvation to utilize stored triacylglycerol in Mycobacterium tuberculosis (2006), J. Biol. Chem., 281, 3866-3875.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.3 additional information starvation induces the enzyme Mycobacterium tuberculosis
3.1.1.3 Triton X-100 activates at 0.1%, inhibits at higher concentrations Mycobacterium tuberculosis
3.1.1.79 potassium acetate enhances activity Mycobacterium tuberculosis
3.1.1.79 SDS slight stimulation up to 2 mM Mycobacterium tuberculosis
3.1.1.79 Sodium acetate enhances activity Mycobacterium tuberculosis
3.1.1.79 Triton X-100 0.1%, stimulates Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.3 diverse genes encoding lipases, e.g. gene Rv3097c or lipY, expression of His-tagged LipY and other lipases in in Escherichia coli strain BL21(DE3), LipY in inclusion bodies, overview Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.3 additional information construction of a gene lipY disruption mutant Mycobacterium tuberculosis

General Stability

EC Number General Stability Organism
3.1.1.79 stable to 4 cycles of freezing at -20°C followed by thawing Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.3 Ca2+
-
 Mycobacterium tuberculosis
3.1.1.3 Co2+
-
 Mycobacterium tuberculosis
3.1.1.3 E-600 nearly complete inhibition of recombinant LipY at 0.0005 mM Mycobacterium tuberculosis
3.1.1.3 Mg2+
-
 Mycobacterium tuberculosis
3.1.1.3 Mn2+
-
 Mycobacterium tuberculosis
3.1.1.3 PMSF 75% inhibition of recombinant LipY at 5 mM Mycobacterium tuberculosis
3.1.1.3 SDS the complete inhibition is partially reversible by Triton X-100, 20% of maximal activity can be recovered Mycobacterium tuberculosis
3.1.1.3 Triton X-100 activates at 0.1%, inhibits at higher concentrations Mycobacterium tuberculosis
3.1.1.3 Zn2+
-
 Mycobacterium tuberculosis
3.1.1.79 CaCl2 50 mM, 47% inhibition Mycobacterium tuberculosis
3.1.1.79 CoCl2 10 mM, 34% inhibition Mycobacterium tuberculosis
3.1.1.79 MgCl2 50 mM, 52% inhibition Mycobacterium tuberculosis
3.1.1.79 MnCl2 50 mM, 45% inhibition Mycobacterium tuberculosis
3.1.1.79 SDS above 2 mM. Inhibition by SDS is partially reversed by Triton X-100 Mycobacterium tuberculosis
3.1.1.79 Triton X-100 above 0.1% Mycobacterium tuberculosis
3.1.1.79 Tween 20 inhibits from 0.1 to 2.0% Mycobacterium tuberculosis
3.1.1.79 ZnCl2 44% inhibition at 10 mM, 99% inhibition at 50 mM Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.3 7.57
-
 triolein pH 8.0, 37°C, recombinant LipY Mycobacterium tuberculosis
3.1.1.79 7.57
-
 triolein
-
 Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.3 K+ activates Mycobacterium tuberculosis
3.1.1.3 Na+ activates Mycobacterium tuberculosis
3.1.1.79 KCl enhances activity Mycobacterium tuberculosis
3.1.1.79 NaCl enhances activity Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.3 45000
-
 x * 45000, recombinant LipY, SDS-PAGE Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.79 additional information Mycobacterium tuberculosis LIPY may be responsible for the utilization of stored triglycerides during dormancy and reactivation of the pathogen Mycobacterium tuberculosis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.3 Mycobacterium tuberculosis P77909 diverse genes encoding lipases with lipY encoding the enzyme with the highest activity, overview
-
3.1.1.3 Mycobacterium tuberculosis H37Rv P77909 diverse genes encoding lipases with lipY encoding the enzyme with the highest activity, overview
-
3.1.1.79 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.3 recombinant His-tagged LipY, solubilization from inclusion bodies after expression in Escherichia coli strain BL21(DE3), purification by nickel affinity chromatography Mycobacterium tuberculosis
3.1.1.79
-
 Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.3 0.00002 0.0027 crude recombinant lipases in Eschericha coli Mycobacterium tuberculosis
3.1.1.3 0.041
-
 crude recombinant LipY in Eschericha coli Mycobacterium tuberculosis

Storage Stability

EC Number Storage Stability Organism
3.1.1.3 -20°C, recombinant purified LipY, highly stable after 4 cycles of freezing and thawing Mycobacterium tuberculosis
3.1.1.3 4°C, recombinant purified LipY, 60 days, highly stable Mycobacterium tuberculosis
3.1.1.79 4°C, stable for 60 days Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.3 diolein + H2O
-
 Mycobacterium tuberculosis 1-oleoyl-sn-glycerol + oleate
-
 ?
3.1.1.3 diolein + H2O
-
 Mycobacterium tuberculosis H37Rv 1-oleoyl-sn-glycerol + oleate
-
 ?
3.1.1.3 triolein + H2O
-
 Mycobacterium tuberculosis diolein + oleate
-
 ?
3.1.1.3 triolein + H2O
-
 Mycobacterium tuberculosis H37Rv diolein + oleate
-
 ?
3.1.1.79 additional information LIPY may be responsible for the utilization of stored triglycerides during dormancy and reactivation of the pathogen Mycobacterium tuberculosis Mycobacterium tuberculosis ?
-
 ?
3.1.1.79 triolein + H2O
-
 Mycobacterium tuberculosis dioleoylglycerol + oleate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.3 ? x * 45000, recombinant LipY, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
3.1.1.3 lipase
-
 Mycobacterium tuberculosis
3.1.1.3 LipY
-
 Mycobacterium tuberculosis
3.1.1.3 More LipY belongs to the HSL family Mycobacterium tuberculosis
3.1.1.3 TG hydrolase
-
 Mycobacterium tuberculosis
3.1.1.79 LipY
-
 Mycobacterium tuberculosis
3.1.1.79 Rv3097c
-
 Mycobacterium tuberculosis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.3 37
-
 assay at Mycobacterium tuberculosis
3.1.1.79 40
-
-
 Mycobacterium tuberculosis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.79 25 50 25°C: about 90% of maximal activity, 50°C: about 40% of maximal activity Mycobacterium tuberculosis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.3 50
-
 15 min, recombinant LipY, stable up to, rapid inactivation above Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.3 8 9 recombinant LipY Mycobacterium tuberculosis
3.1.1.79 8 9
-
 Mycobacterium tuberculosis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.79 7 10 pH 7: about 60% of maximal activity, pH 10.: about 50% of maximal activity Mycobacterium tuberculosis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.3 Mycobacterium tuberculosis
-
-
 4.5